PMID- 24417405
OWN - NLM
STAT- MEDLINE
DCOM- 20140904
LR  - 20190816
IS  - 1091-7691 (Electronic)
IS  - 0895-8378 (Linking)
VI  - 26
IP  - 1
DP  - 2014 Jan
TI  - Host and environmental factors affect pulmonary responses measured in 
      bronchoalveolar lavage.
PG  - 30-5
LID - 10.3109/08958378.2013.851304 [doi]
AB  - CONTEXT: Bronchoscopy with bronchoalveolar lavage (BAL) is used to measure 
      pulmonary effects in inhalational exposure studies. OBJECTIVES: To determine how 
      host and background environmental factors may affect pulmonary responses in BAL. 
      MATERIALS AND METHODS: We retrospectively analyzed 77 healthy non-smoking 
      volunteers (38 males and 39 females, age 18-35) who participated in a 
      bronchoscopy study to donate cells for in vitro studies. BAL was performed by 
      lavaging one subsegment of both the lingular segment of the left upper lobe and 
      the right middle lobe with 250 ml of sterile normal saline each. We obtained 
      temperature, relative humidity, ambient O3, PM2.5 and PM10 levels from monitor 
      stations in Durham area in North Carolina. We correlated concentrations of 
      leptin, adiponectin, monocyte chemotactic protein-1 (MCP-1), interleukin (IL)-8, 
      ferritin and total lavaged cells in BAL samples with body mass index (BMI), age, 
      ambient O3, PM2.5, PM10, temperature and relative humidity. RESULTS: Increased 
      BMI was associated with higher lavage leptin. Males had higher MCP-1 and total 
      lavaged cells than females. Average PM2.5, PM10 and O3 concentrations before 
      bronchoscopy were 13.7 microg/m(3), 21.2 microg/m(3) and 0.029 ppm, respectively. Using 
      stepwise multiple linear regression, we found positive associations of MCP-1 with 
      BMI, and of total lavaged cells with humidity and O3. There were inverse 
      associations of IL-8 and total lavaged cells with temperature. DISCUSSION AND 
      CONCLUSIONS: Background environmental and host factors may affect some pulmonary 
      responses to ambient pollutants. Interpretation of pulmonary effects in 
      inhalational exposure studies may need to consider the effects of some host and 
      environmental factors.
FAU - Huang, Jennifer J
AU  - Huang JJ
AD  - Human Studies Division, National Health Effects Research Laboratory, US 
      Environmental Protection Agency , Chapel Hill, NC , USA and.
FAU - Soukup, Joleen M
AU  - Soukup JM
FAU - Ghio, Andrew J
AU  - Ghio AJ
FAU - Huang, Yuh-Chin T
AU  - Huang YC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
RN  - 0 (ADIPOQ protein, human)
RN  - 0 (Adiponectin)
RN  - 0 (Air Pollutants)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Leptin)
RN  - 0 (Particulate Matter)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 66H7ZZK23N (Ozone)
RN  - 9007-73-2 (Ferritins)
SB  - IM
MH  - Adiponectin/analysis
MH  - Adolescent
MH  - Adult
MH  - Air Pollutants/analysis/*toxicity
MH  - Body Mass Index
MH  - Bronchoalveolar Lavage Fluid/*chemistry/*cytology
MH  - Cell Count
MH  - Chemokine CCL2/analysis
MH  - Environmental Exposure
MH  - Female
MH  - Ferritins/analysis
MH  - Humans
MH  - Humidity
MH  - Interleukin-8/analysis
MH  - Leptin/analysis
MH  - Male
MH  - Ozone/analysis/*toxicity
MH  - Particulate Matter/analysis/*toxicity
MH  - Temperature
MH  - Tumor Necrosis Factor-alpha/analysis
MH  - Young Adult
EDAT- 2014/01/15 06:00
MHDA- 2014/09/05 06:00
CRDT- 2014/01/15 06:00
PHST- 2014/01/15 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/09/05 06:00 [medline]
AID - 10.3109/08958378.2013.851304 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2014 Jan;26(1):30-5. doi: 10.3109/08958378.2013.851304.