PMID- 24418538
OWN - NLM
STAT- MEDLINE
DCOM- 20140407
LR  - 20231213
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 449
DP  - 2014 Jan 20
TI  - The respiratory syncytial virus (RSV) nonstructural proteins mediate RSV 
      suppression of glucocorticoid receptor transactivation.
PG  - 62-9
LID - S0042-6822(13)00627-2 [pii]
LID - 10.1016/j.virol.2013.11.014 [doi]
AB  - Respiratory syncytial virus (RSV)-induced bronchiolitis in infants is not 
      responsive to glucocorticoids. We have shown that RSV infection impairs 
      glucocorticoid receptor (GR) function. In this study, we have investigated the 
      mechanism by which RSV impairs GR function. We have shown that RSV repression of 
      GR-induced transactivation is not mediated through a soluble autocrine factor. 
      Knock-down of mitochondrial antiviral signaling protein (MAVS), but not retinoic 
      acid-inducible gene 1 (RIG-I) or myeloid differentiation primary response gene 88 
      (MyD88), impairs GR-mediated gene activation even in mock-infected cells. 
      Over-expression of the RSV nonstructural protein NS1, but not NS2, impairs 
      glucocorticoid-induced transactivation and viruses deleted in NS1 and/or NS2 are 
      unable to repress glucocorticoid-induction of the known GR regulated gene 
      glucocorticoid-inducible leucine zipper (GILZ). These data suggest that the RSV 
      nonstructural proteins mediate RSV repression of GR-induced transactivation and 
      that inhibition of the nonstructural proteins may be a viable target for therapy 
      against RSV-related disease.
CI  - (c) 2013 Published by Elsevier Inc.
FAU - Webster Marketon, Jeanette I
AU  - Webster Marketon JI
AD  - Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of 
      Internal Medicine, Wexner Medical Center at The Ohio State University, Columbus, 
      OH 43210, United States; Institute for Behavioral Medicine Research, Wexner 
      Medical Center at The Ohio State University, Columbus, OH 43210, United States. 
      Electronic address: jeanette.marketon@gmail.com.
FAU - Corry, Jacqueline
AU  - Corry J
AD  - Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of 
      Internal Medicine, Wexner Medical Center at The Ohio State University, Columbus, 
      OH 43210, United States. Electronic address: 
      Jacqueline.corry@nationwidechildrens.org.
FAU - Teng, Michael N
AU  - Teng MN
AD  - Joy McCann Culverhouse Airway Disease Research Center, Division of Allergy and 
      Immunology, Department of Internal Medicine, Morsani College of Medicine, 
      University of South Florida, Tampa, FL 33612, United States.
LA  - eng
GR  - R01 AI081977/AI/NIAID NIH HHS/United States
GR  - R01AI081977/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20131126
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Glucocorticoids)
RN  - 0 (MAVS protein, human)
RN  - 0 (NS2 protein, human respiratory syncytial virus)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 3.6.1.- (RIGI protein, human)
RN  - EC 3.6.4.13 (DEAD Box Protein 58)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/metabolism
MH  - DEAD Box Protein 58
MH  - DEAD-box RNA Helicases/genetics/metabolism
MH  - Down-Regulation
MH  - Glucocorticoids/metabolism
MH  - Humans
MH  - Receptors, Glucocorticoid/genetics/*metabolism
MH  - Receptors, Immunologic
MH  - Respiratory Syncytial Virus Infections/genetics/*metabolism/virology
MH  - Respiratory Syncytial Virus, Human/genetics/*metabolism
MH  - *Transcriptional Activation
MH  - Viral Nonstructural Proteins/genetics/*metabolism
PMC - PMC3904736
MID - NIHMS544859
OTO - NOTNLM
OT  - Gene regulation
OT  - Glucocorticoid
OT  - Nonstructural proteins
OT  - Respiratory syncytial virus
EDAT- 2014/01/15 06:00
MHDA- 2014/04/08 06:00
PMCR- 2015/01/20
CRDT- 2014/01/15 06:00
PHST- 2013/06/06 00:00 [received]
PHST- 2013/07/12 00:00 [revised]
PHST- 2013/11/06 00:00 [accepted]
PHST- 2014/01/15 06:00 [entrez]
PHST- 2014/01/15 06:00 [pubmed]
PHST- 2014/04/08 06:00 [medline]
PHST- 2015/01/20 00:00 [pmc-release]
AID - S0042-6822(13)00627-2 [pii]
AID - 10.1016/j.virol.2013.11.014 [doi]
PST - ppublish
SO  - Virology. 2014 Jan 20;449:62-9. doi: 10.1016/j.virol.2013.11.014. Epub 2013 Nov 
      26.