PMID- 2442033
OWN - NLM
STAT- MEDLINE
DCOM- 19871014
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 221
IP  - 1
DP  - 1987 Aug 31
TI  - Are Ca2+ channels in neutrophils activated by a rise in cytosolic free Ca2+?
PG  - 95-100
AB  - It was recently suggested that the opening of neutrophil plasma membrane Ca2+ 
      channels by chemotactic agents is mediated by a rise in free cytosolic Ca2+ 
      concentration ([Ca2+]i). This hypothesis was tested in human cells monitoring 
      [Ca2+]i with the indicator indo-1. In cells loaded with the Ca2+-chelating agent 
      bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetate, transmembrane Ca2+ uptake could 
      be stimulated by formyl-methionyl-leucyl-phenylalanine (fMLP) even when [Ca2+]i 
      was at or below the resting level. In contrast, simply elevating [Ca2+]i in 
      unstimulated cells failed to increase transmembrane uptake. It was concluded 
      either that Ca2+ uptake across the plasma membrane is activated directly by the 
      formation of the chemotactic factor-receptor complex or, more likely, that a 
      transduction mechanism distinct from changes in [Ca2+]i is involved.
FAU - Nasmith, P E
AU  - Nasmith PE
FAU - Grinstein, S
AU  - Grinstein S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Indoles)
RN  - 0 (Ion Channels)
RN  - 526U7A2651 (Egtazic Acid)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)
RN  - N18RMK75W1 (indo-1)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*blood
MH  - Cell Membrane/metabolism
MH  - Egtazic Acid/analogs & derivatives/pharmacology
MH  - Fluorescent Dyes
MH  - Humans
MH  - Indicators and Reagents
MH  - Indoles
MH  - Ion Channels/drug effects/*metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*metabolism
MH  - Spectrometry, Fluorescence
EDAT- 1987/08/31 00:00
MHDA- 1987/08/31 00:01
CRDT- 1987/08/31 00:00
PHST- 1987/08/31 00:00 [pubmed]
PHST- 1987/08/31 00:01 [medline]
PHST- 1987/08/31 00:00 [entrez]
AID - 0014-5793(87)80359-9 [pii]
AID - 10.1016/0014-5793(87)80359-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1987 Aug 31;221(1):95-100. doi: 10.1016/0014-5793(87)80359-9.