PMID- 24422953
OWN - NLM
STAT- MEDLINE
DCOM- 20140919
LR  - 20201209
IS  - 1440-1827 (Electronic)
IS  - 1320-5463 (Linking)
VI  - 63
IP  - 12
DP  - 2013 Dec
TI  - Reappraisal of TLE-1 immunohistochemical staining and molecular detection of 
      SS18-SSX fusion transcripts for synovial sarcoma.
PG  - 573-80
LID - 10.1111/pin.12113 [doi]
AB  - The aim of the present study was to re-evaluate TLE-1 staining and the molecular 
      detection methods of SS18-SSX transcripts for synovial sarcoma. We analyzed TLE-1 
      expression in 50 molecularly confirmed synovial sarcomas and 85 other soft tissue 
      tumors with three previously published scoring systems. In the present study, 39 
      to 43 synovial sarcomas showed TLE-1 nuclear staining, whereas 9-15 of 85 other 
      soft tissue tumors showed TLE-1 staining (P < 0.0001). The specificities of 
      strong TLE-1 staining were 100%, 97.6% and 98.8%. The positive likelihood ratio 
      of moderate and strong TLE-1 nuclear expression was >10 in all three scoring 
      systems. There was no difference in TLE-1 staining between different subtypes of 
      synovial sarcoma (P > 0.05). Based on a comparison between conventional reverse 
      transcription (RT)-polymerase chain reaction (PCR) and fluorescence in situ 
      hybridization (FISH), quantitative RT-PCR is a more sensitive method than 
      conventional RT-PCR and FISH to detect t(X;18). A positive correlation between 
      TLE-1 staining and SS18-SSX translocation was detected by conventional PCR 
      (P < 0.05). In conclusion, although all three scoring systems could differentiate 
      synovial sarcoma from other soft tissue tumors, diffuse moderate to severe 
      intensity tumors showed the highest specificity in the diagnosis of synovial 
      sarcoma.
CI  - (c) 2013 The Authors. Pathology International (c) 2013 Japanese Society of Pathology 
      and Wiley Publishing Asia Pty Ltd.
FAU - Chuang, Huei-Chieh
AU  - Chuang HC
AD  - Department of Pathology, Chang Gung Memorial Hospital, Chang Gung University 
      School of Medicine, Kwei-Shan, Tao-Yuan, Taiwan.
FAU - Hsu, Sheng-Chi
AU  - Hsu SC
FAU - Huang, Chung-Guei
AU  - Huang CG
FAU - Hsueh, Swei
AU  - Hsueh S
FAU - Ng, Kwai-Fong
AU  - Ng KF
FAU - Chen, Tse-Ching
AU  - Chen TC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (Co-Repressor Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (TLE1 protein, human)
SB  - IM
MH  - Co-Repressor Proteins
MH  - Diagnosis, Differential
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Repressor Proteins/genetics/*metabolism
MH  - Sarcoma, Synovial/*diagnosis/genetics/metabolism
MH  - Soft Tissue Neoplasms/*diagnosis/genetics/metabolism
MH  - *Translocation, Genetic
OTO - NOTNLM
OT  - 18)
OT  - SS18
OT  - SSX
OT  - TLE-1
OT  - immunohistochemistry
OT  - molecular diagnosis
OT  - scoring system
OT  - synovial sarcoma
OT  - t(X
EDAT- 2014/01/16 06:00
MHDA- 2014/09/23 06:00
CRDT- 2014/01/16 06:00
PHST- 2013/06/12 00:00 [received]
PHST- 2013/10/27 00:00 [accepted]
PHST- 2014/01/16 06:00 [entrez]
PHST- 2014/01/16 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
AID - 10.1111/pin.12113 [doi]
PST - ppublish
SO  - Pathol Int. 2013 Dec;63(12):573-80. doi: 10.1111/pin.12113.