PMID- 24424397
OWN - NLM
STAT- MEDLINE
DCOM- 20140909
LR  - 20211021
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 33
IP  - 3
DP  - 2014 Mar
TI  - Human single-chain variable fragment antibody inhibits macrophage migration 
      inhibitory factor tautomerase activity.
PG  - 515-22
LID - 10.3892/ijmm.2014.1622 [doi]
AB  - Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine, 
      secreted from a variety of immune cells, that regulates innate and adaptive 
      immune responses. Elevation of MIF levels in plasma correlates with the severity 
      of inflammatory diseases in humans. Inhibition of MIF or its tautomerase activity 
      ameliorates disease severity by reducing inflammatory responses. In this study, 
      the human single-chain variable fragment (HuScFv) antibody specific to MIF was 
      selected from the human antibody phage display library by using purified 
      recombinant full-length human MIF (rMIF) as the target antigen. Monoclonal HuScFv 
      was produced from phage-transformed bacteria and tested for their binding 
      activities to rMIF by indirect enzyme-linked immunosorbent assay as well as to 
      native MIF by western blot analysis and immunofluorescence assay. The HuScFv with 
      highest binding signal to rMIF also inhibited the tautomerase activities of both 
      rMIF and native MIF in human monoblastic leukemia (U937) cells in a 
      dose-dependent manner. Mimotope searching and molecular docking concordantly 
      demonstrated that the HuScFv interacted with Lys32 and Ile64 in the MIF 
      tautomerase active site. To the best of our knowledge, this is the first study to 
      focus on MIF-specific fully-human antibody fragment with a tautomerase-inhibitory 
      effect that has potential to be developed as anti-inflammatory biomolecules for 
      human use.
FAU - Tarasuk, Mayuri
AU  - Tarasuk M
AD  - Division of Molecular Medicine, Department of Research and Development, Faculty 
      of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Poungpair, Ornnuthchar
AU  - Poungpair O
AD  - Division of Molecular Medicine, Department of Research and Development, Faculty 
      of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Ungsupravate, Duangporn
AU  - Ungsupravate D
AD  - Division of Molecular Medicine, Department of Research and Development, Faculty 
      of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Bangphoomi, Kunan
AU  - Bangphoomi K
AD  - Department of Biochemistry, Faculty of Sciences, Kasetsart University, Bangkok, 
      Thailand.
FAU - Chaicumpa, Wanpen
AU  - Chaicumpa W
AD  - Laboratory for Research and Technology Development, Department of Parasitology, 
      Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
FAU - Yenchitsomanus, Pa-Thai
AU  - Yenchitsomanus PT
AD  - Division of Molecular Medicine, Department of Research and Development, Faculty 
      of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140113
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Macrophage Migration-Inhibitory Factors)
RN  - 0 (Single-Chain Antibodies)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.2.1 (MIF protein, human)
SB  - IM
MH  - Anti-Inflammatory Agents/*administration & dosage
MH  - Catalytic Domain
MH  - Cell Line, Tumor
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - *Immunity, Innate
MH  - Intramolecular Oxidoreductases/antagonists & inhibitors/immunology/*metabolism
MH  - Leukemia/*drug therapy/immunology
MH  - Macrophage Migration-Inhibitory Factors/antagonists & 
      inhibitors/immunology/*metabolism
MH  - Protein Binding/immunology
MH  - Single-Chain Antibodies/*administration & dosage/metabolism
PMC - PMC3926510
EDAT- 2014/01/16 06:00
MHDA- 2014/09/10 06:00
PMCR- 2014/01/13
CRDT- 2014/01/16 06:00
PHST- 2013/09/13 00:00 [received]
PHST- 2013/12/24 00:00 [accepted]
PHST- 2014/01/16 06:00 [entrez]
PHST- 2014/01/16 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
PHST- 2014/01/13 00:00 [pmc-release]
AID - ijmm-33-03-0515 [pii]
AID - 10.3892/ijmm.2014.1622 [doi]
PST - ppublish
SO  - Int J Mol Med. 2014 Mar;33(3):515-22. doi: 10.3892/ijmm.2014.1622. Epub 2014 Jan 
      13.