PMID- 24427326
OWN - NLM
STAT- MEDLINE
DCOM- 20140905
LR  - 20220318
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 7
IP  - 1
DP  - 2014
TI  - MiR-19a is correlated with prognosis and apoptosis of laryngeal squamous cell 
      carcinoma by regulating TIMP-2 expression.
PG  - 56-63
AB  - MiRNAs are small, noncoding RNA molecules that act as posttranscriptional 
      regulators of gene expression and function as important regulators in 
      cancer-related processes. The miR-19a is overexpressed in various cancers and has 
      been causally related to cellular proliferation and growth. To determine whether 
      miR-19a plays a role in laryngeal squamous cell carcinoma (LSCC), we used 
      quantitative real time PCR to detect miR-19a expression in LSCC tissues. We found 
      that miR-19a is overexpressed in LSCC and correlated with neck nodal metastasis, 
      poor differentiation and advanced stage. Statistical analysis suggests that 
      higher level of miR-19a was associated with reduced overall survival. In vitro 
      functional study showed that inhibition of miR-19a by antisense oligonucleotides 
      (ASO) led to apoptosis and reduction of cell proliferation in LSCC cells. 
      Furthermore, growth of LSCC xenograft tumors was significantly suppressed by 
      repeated injection of ASO-miR-19a lentivirus. The TUNEL stain and transmission 
      electron microscopy also detected increased apoptotic cells in ASO-miR-19a 
      treated LSCC xenografts. In addition, both realtime PCR and western blot showed 
      ASO-miR-19a can upregulate TIMP-2 expression and this suggests miR-19a is related 
      with TIMP-2 pathway in LSCC cells. Taken together, these data suggest that 
      miR-19a plays an oncogenic role in the progression of LSCC, and may serve as a 
      biomarker or therapeutic target for patients with LSCC.
FAU - Wu, Tian-Yi
AU  - Wu TY
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University Harbin, China.
FAU - Zhang, Tian-Hong
AU  - Zhang TH
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated 
      Hospital, Harbin Medical University Harbin, China.
FAU - Qu, Ling-Mei
AU  - Qu LM
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University Harbin, China.
FAU - Feng, Jia-Peng
AU  - Feng JP
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University Harbin, China.
FAU - Tian, Lin-Li
AU  - Tian LL
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University Harbin, China.
FAU - Zhang, Bing-Hui
AU  - Zhang BH
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University Harbin, China.
FAU - Li, Dan-Dan
AU  - Li DD
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University Harbin, China.
FAU - Sun, Ya-Nan
AU  - Sun YN
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University Harbin, China.
FAU - Liu, Ming
AU  - Liu M
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University Harbin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131215
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (MIRN19 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (TIMP2 protein, human)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/pathology
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Heterografts
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Kaplan-Meier Estimate
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*genetics
MH  - Microscopy, Electron, Transmission
MH  - Prognosis
MH  - Real-Time Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinase-2/*biosynthesis/genetics
PMC - PMC3885460
OTO - NOTNLM
OT  - LSCC
OT  - TIMP-2
OT  - apoptosis
OT  - miR-19a
EDAT- 2014/01/16 06:00
MHDA- 2014/09/06 06:00
PMCR- 2013/12/15
CRDT- 2014/01/16 06:00
PHST- 2013/10/25 00:00 [received]
PHST- 2013/11/27 00:00 [accepted]
PHST- 2014/01/16 06:00 [entrez]
PHST- 2014/01/16 06:00 [pubmed]
PHST- 2014/09/06 06:00 [medline]
PHST- 2013/12/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2013 Dec 15;7(1):56-63. eCollection 2014.