PMID- 24427767
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140115
LR  - 20211021
IS  - 2287-3651 (Print)
IS  - 2287-366X (Electronic)
IS  - 2287-3651 (Linking)
VI  - 3
IP  - 1
DP  - 2014 Jan
TI  - Development of porcine respiratory and reproductive syndrome virus replicon 
      vector for foot-and-mouth disease vaccine.
PG  - 100-9
LID - 10.7774/cevr.2014.3.1.100 [doi]
AB  - PURPOSE: Foot-and-mouth disease (FMD) is an economically important global animal 
      disease. To control FMD virus (FMDV) outbreaks, a lot of different novel 
      approaches have been attempted. In this study, we proposed a novel porcine 
      reproductive and respiratory syndrome virus (PRRSV) as a replicon vector to 
      express FMDV structural protein. MATERIALS AND METHODS: PRRSV infectious clone 
      (PRRSVK418DM) was used to develop an expression vector through the reverse 
      genetic manipulation of PRRSV; FMDVP12A3C gene of serotype O was synthesized and 
      used for an antigen. MARC-145 cells (African green monkey kidney epithelial cell 
      line) were used for electroporation mediated transfection. The transfection or 
      the expression of P12A3C and N protein of PRRSV was analyzed by either replicon 
      containing PRRSV alone or by co-infection of helper PRRSV. RESULTS: We 
      constructed PRRSVK418DM replicon vector containing FMDVP12A3C, and genome 
      sequences were confirmed by subsequent sequence analysis. In vitro expression of 
      P12A3C and PRRSV N protein was confirmed by immunofluorescence antibody assay 
      using antibodies specific for PRRSV N protein (anti-PRRSV N MAb), FMDV-VP1 
      (anti-VP1 MAb). CONCLUSION: The results indicate that PRRSV replicon vector can 
      be a promising novel vector system to control FMDV and useful for vaccine 
      development in the future.
FAU - Jeeva, Subbiah
AU  - Jeeva S
AD  - College of Veterinary Medicine and Veterinary Science Research institute, Konkuk 
      University, Seoul, Korea.
FAU - Lee, Jung-Ah
AU  - Lee JA
AD  - College of Veterinary Medicine and Veterinary Science Research institute, Konkuk 
      University, Seoul, Korea.
FAU - Park, Seung-Yong
AU  - Park SY
AD  - College of Veterinary Medicine and Veterinary Science Research institute, Konkuk 
      University, Seoul, Korea.
FAU - Song, Chang-Seon
AU  - Song CS
AD  - College of Veterinary Medicine and Veterinary Science Research institute, Konkuk 
      University, Seoul, Korea.
FAU - Choi, In-Soo
AU  - Choi IS
AD  - College of Veterinary Medicine and Veterinary Science Research institute, Konkuk 
      University, Seoul, Korea.
FAU - Lee, Joong-Bok
AU  - Lee JB
AD  - College of Veterinary Medicine and Veterinary Science Research institute, Konkuk 
      University, Seoul, Korea.
LA  - eng
PT  - Journal Article
DEP - 20131218
PL  - Korea (South)
TA  - Clin Exp Vaccine Res
JT  - Clinical and experimental vaccine research
JID - 101592344
PMC - PMC3890444
OTO - NOTNLM
OT  - FMDV
OT  - Genetic vectors
OT  - PRRSV
OT  - Replicon
COIS- No potential conflict of interest relevant to this article was reported.
EDAT- 2014/01/16 06:00
MHDA- 2014/01/16 06:01
PMCR- 2014/01/01
CRDT- 2014/01/16 06:00
PHST- 2013/10/20 00:00 [received]
PHST- 2013/11/10 00:00 [revised]
PHST- 2013/11/14 00:00 [accepted]
PHST- 2014/01/16 06:00 [entrez]
PHST- 2014/01/16 06:00 [pubmed]
PHST- 2014/01/16 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - 10.7774/cevr.2014.3.1.100 [doi]
PST - ppublish
SO  - Clin Exp Vaccine Res. 2014 Jan;3(1):100-9. doi: 10.7774/cevr.2014.3.1.100. Epub 
      2013 Dec 18.