PMID- 24428604
OWN - NLM
STAT- MEDLINE
DCOM- 20150615
LR  - 20191112
IS  - 2212-3946 (Electronic)
IS  - 1574-888X (Linking)
VI  - 9
IP  - 4
DP  - 2014
TI  - Induced human bone marrow stromal cells differentiate into neural cells by bFGF 
      and cocultured with olfactory ensheathing cells.
PG  - 291-6
AB  - Bone marrow stromal cells (BMSCs) were considered as one of the strongest 
      candidates for cell transplantations to treat neurological disorders. Previously, 
      we had showed that BMSCs isolated from rats could be induced to differentiate 
      into neural cells being cocultured with olfactory ensheathing cells (OECs). In 
      this study, we further demonstrated the neural differentiation of human BMSCs 
      (hBMSCs) when cocultured with OECs and daily supplement of bFGF (basic fibroblast 
      growth factor). Transwell culture dishes with a 0.4-mm pore size were used to 
      coculture hBMSCs and OECs. At different time points (12h, 24h, 3d, 7d, 14d), the 
      induced hBMSCs were morphologically observed and performed immunocytofluorescence 
      and quantitative RT-PCR (qRT-PCR). The number of neural markers-positive cells 
      significantly increased after coculture, and gene expression of NSE, 
      beta-III-tubulin, MAP2, GFAP also dramatically increased. Our study suggested that 
      hBMSCs could be induced into neuron-like cells under conditions of coculture with 
      OECs and daily supplement of bFGF. The differentiated autologous hBMSCs had a 
      great potential for transplantation to treat CNS lesion.
FAU - Ni, Wen-Fei
AU  - Ni WF
FAU - Wu, Ai-Min
AU  - Wu AM
FAU - Li, Qing-Long
AU  - Li QL
FAU - Huang, Zhe-Yu
AU  - Huang ZY
FAU - Xu, Hua-Zi
AU  - Xu HZ
FAU - Yin, Li-Hui
AU  - Yin LH
AD  - Institute of Medical Science, The First Affiliated Hospital of Wenzhou Medical 
      University, China. lihuiyin1976@yeah.net.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United Arab Emirates
TA  - Curr Stem Cell Res Ther
JT  - Current stem cell research & therapy
JID - 101272517
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - *Cell Differentiation
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Fibroblast Growth Factor 2/*physiology
MH  - Gene Expression
MH  - Humans
MH  - Mesenchymal Stem Cells/*physiology
MH  - Neurons/physiology
MH  - Olfactory Mucosa/cytology
EDAT- 2014/01/17 06:00
MHDA- 2015/06/16 06:00
CRDT- 2014/01/17 06:00
PHST- 2013/12/06 00:00 [received]
PHST- 2014/01/03 00:00 [revised]
PHST- 2014/01/10 00:00 [accepted]
PHST- 2014/01/17 06:00 [entrez]
PHST- 2014/01/17 06:00 [pubmed]
PHST- 2015/06/16 06:00 [medline]
AID - CSCRT-EPUB-58636 [pii]
AID - 10.2174/1574888x09666140115114350 [doi]
PST - ppublish
SO  - Curr Stem Cell Res Ther. 2014;9(4):291-6. doi: 10.2174/1574888x09666140115114350.