PMID- 24428989
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20140116
IS  - 1001-9391 (Print)
IS  - 1001-9391 (Linking)
VI  - 32
IP  - 1
DP  - 2014 Jan
TI  - [Tumor necrosis factor-alpha and NF-kappaB play a role in macrophage-like THP-1 cells 
      promoting coal tar pitch extract-induced tumorigenic transformation of human 
      bronchial epithelial cells].
PG  - 38-43
AB  - OBJECTIVE: To characterize the role of tumor necrosis factor-alpha (TNF-alpha) and NF-kappaB 
      play a role in macrophage-like THP-1 cells promoting coal tar pitch extract 
      (CTPE)-induced tumorigenic transformation of human bronchial epithelial cells 
      (BEAS-2B). METHODS: From passage 10, CTPE-induced BEAS-2B cells cocultured with 
      THP-1 cells were treated with NF-kappaB inhibitor-Pyrrolidine dithiocarbamate (PDTC) 
      every 3 passages and TNF-alpha antibody every passage. Alterations of cell cycle, 
      karyotype and colony formation in soft agar of BEAS-2B cells at passages 20, 
      indicative of tumorigenicity, were determined, respectively. In addition, mRNA 
      and protein levels of TNF receptor associated factor2 (TRAF2) and Cyclin D1 in 
      BEAS-2B cells were measured with Real Time-PCR and Western blot, respectively. 
      RESULTS: The percentages of S-phase BEAS-2B cells at passage 20 in PDTC group and 
      TNF-alpha antibody group were (33.97+/-2.16)% and (34.29+/-2.04)% respectively, which 
      were less than that in Co-culture+CTPE group of 20th passage [(44.46+/-0.83)%], P < 
      0.05; The number of cells with aneuploidy in 100 cells in 20th passage PDTC group 
      and TNF-alpha antibody group were 40 and 37, and there were significantly different 
      when comparing to that of 20th passage Co-culture+CTPE group (75); The number of 
      colony formation and the rate of colony formation of BEAS-2B cells in soft agar 
      at passage 20 in PDTC group were (15.17+/-2.48) and (1.51 per thousand+/-0.25 per thousand), (13.33+/-2.58)and 
      (1.33 per thousand+/-0.26 per thousand) in TNF-alpha antibody group, which were less that those in 20th passage 
      Co-culture+CTPE group [(172.33+/-12.09) and (17.23 per thousand+/-1.20 per thousand)], P < 0.05; at the same 
      time, the mRNA and protein levels of TRAF2 and Cyclin D1 in BEAS-2B cells were 
      decreased after PDTC and TNF-alpha antibody treatment. CONCLUSION: TNF-alpha and NF-kappaB 
      could play an important role in THP-1 cells promoting coal tar pitch 
      extract-induced tumorigenic transformation of BEAS-2B cells by influencing the 
      expression of TRAF2 and Cyclin D1.
FAU - Feng, Feifei
AU  - Feng F
AD  - Department of Toxicology, College of Public Health, Zhengzhou University, 
      Zhengzhou 450001, China.
FAU - Zhang, Qiao
AU  - Zhang Q
AD  - Department of Toxicology, College of Public Health, Zhengzhou University, 
      Zhengzhou 450001, China.
FAU - Zhou, Fanjing
AU  - Zhou F
AD  - Department of Toxicology, College of Public Health, Zhengzhou University, 
      Zhengzhou 450001, China.
FAU - Wu, Yongjun
AU  - Wu Y
AD  - Department of Toxicology, College of Public Health, Zhengzhou University, 
      Zhengzhou 450001, China.
FAU - Wu, Yiming
AU  - Wu Y
AD  - Department of Toxicology, College of Public Health, Zhengzhou University, 
      Zhengzhou 450001, China. E-mail: wuym369@163.com.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
JT  - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng 
      zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
JID - 8410840
RN  - 0 (CCND1 protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 136601-57-5 (Cyclin D1)
RN  - 8007-45-2 (Coal Tar)
SB  - IM
MH  - Bronchi/cytology
MH  - Cell Line
MH  - Cell Transformation, Neoplastic/*drug effects
MH  - Coal Tar/*toxicity
MH  - Cyclin D1/metabolism
MH  - Epithelial Cells/cytology
MH  - Humans
MH  - Macrophages/*cytology
MH  - NF-kappa B/*metabolism
MH  - TNF Receptor-Associated Factor 2/metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2014/01/17 06:00
MHDA- 2015/04/10 06:00
CRDT- 2014/01/17 06:00
PHST- 2014/01/17 06:00 [entrez]
PHST- 2014/01/17 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
PST - ppublish
SO  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2014 Jan;32(1):38-43.