PMID- 24432635
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 31
IP  - 10
DP  - 2013 Oct
TI  - [Determination of three azaspiracids in edible shellfishes by QuEChERS method 
      combined with ultra high performance liquid chromatography-tandem mass 
      spectrometry].
PG  - 939-45
AB  - An ultra high performance liquid chromatography-tandem mass spectrometric method 
      (UHPLC-MS/MS) was developed for the simultaneous determination of three natural 
      forms of azaspiracids (AZA-1, AZA-2 and AZA-3) in edible shellfishes such as 
      mussels, oysters, clams and scallops. The samples were homogeneously extracted 
      with acetonitrile-water (85: 15, v/v). The resultant supernatants were purified 
      with QuEChERS method and filtrated by 0.2 microm microporous filters. The 
      separation was performed on an Agilent ZORBAX Eclipse Plus C18 column (100 mm x 
      2.1 mm, 1.8 microm) with the gradient elution using acetonitrile/water 
      (containing 5 mmol/L ammonium acetate and 0.1% formic acid) as mobile phases. The 
      three azaspiracids were detected using positive electrospray ionization (ESI +) 
      followed with multiple reaction monitoring (MRM), and quantified by external 
      standard calibration method. The calibration curves showed good linearity in the 
      range of 1-100 microg/kg with the correlation coefficients (r2) greater than 
      0.995. The limits of quantification (LOQ, S/N = 10) were 1.0 microg/kg for all 
      the three AZAs. The average recoveries of azaspiracids spiked in the matrix at 
      the levels of 10, 20 and 50 microg/kg ranged from 71% to 108%. The relative 
      standard deviations (RSDs) of inter-day and intra-day determinations were less 
      than 10% (n = 6). The samples from several areas within and outside of China were 
      screened, and some of the samples showed positive for azaspiracids. The developed 
      method is easy to operate, very reproducible, sensitive, and efficient. It can be 
      applied to the determination of the three forms of AZAs in the edible shellfishes 
      as well shellfish products.
FAU - Han, Shen
AU  - Han S
AD  - Inspection and Quarantine Technical Center of Beijing Entry-Exit Inspection and 
      Quarantine Bureau, Beijing 100026, China.
FAU - Wang, Peiyue
AU  - Wang P
AD  - Inspection and Quarantine Technical Center of Beijing Entry-Exit Inspection and 
      Quarantine Bureau, Beijing 100026, China.
FAU - Liu, Ying
AU  - Liu Y
AD  - Inspection and Quarantine Technical Center of Beijing Entry-Exit Inspection and 
      Quarantine Bureau, Beijing 100026, China.
FAU - Gu, Jin
AU  - Gu J
AD  - Inspection and Quarantine Technical Center of Beijing Entry-Exit Inspection and 
      Quarantine Bureau, Beijing 100026, China.
FAU - Lu, Meiling
AU  - Lu M
AD  - Agilent Technologies (China) Co., Ltd., Beijing 100102, China.
FAU - Wang, Jinhua
AU  - Wang J
AD  - Inspection and Quarantine Technical Center of Beijing Entry-Exit Inspection and 
      Quarantine Bureau, Beijing 100026, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Marine Toxins)
RN  - 0 (Spiro Compounds)
RN  - 0 (azaspiracid)
SB  - IM
MH  - *Chromatography, High Pressure Liquid
MH  - Food Contamination/*analysis
MH  - Marine Toxins/*analysis
MH  - *Shellfish
MH  - Spiro Compounds/*analysis
MH  - *Tandem Mass Spectrometry
EDAT- 2014/01/18 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/01/18 06:00
PHST- 2014/01/18 06:00 [entrez]
PHST- 2014/01/18 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.3724/sp.j.1123.2013.03042 [doi]
PST - ppublish
SO  - Se Pu. 2013 Oct;31(10):939-45. doi: 10.3724/sp.j.1123.2013.03042.