PMID- 24432636
OWN - NLM
STAT- MEDLINE
DCOM- 20150708
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 31
IP  - 10
DP  - 2013 Oct
TI  - [Simultaneous determination of penicillin and their major enzymatic metabolites 
      in milk and milk powder by high performance liquid chromatography-tandem mass 
      spectrometry].
PG  - 946-53
AB  - A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS) 
      method has been developed for the determination of eight compounds in milk and 
      milk powder. They are four penicillins (penicillin G, penicillin V, amoxicillin 
      and ampicillin) and four major beta-lactamase enzymatic metabolites of them 
      (penilloic acid G, penilloic acid V, amoxiilloic acid and ampilloic acid). The 
      compounds were extracted from the samples with acetonitrile and water, cleaned-up 
      by HLB solid-phase extraction cartridges, and then detected by HPLC-MS/MS and 
      quantified by external standard method. The linearities were satisfactory with 
      the correlation coefficients > 0.99 at the mass concentrations ranging from 4 
      microg/L to 200 microg/L for penicillins and from 10 microg/L to 500 microg/L for 
      enzymatic metabolites. The limits of detection and the limits of quantification 
      were 5-50 microg/kg (S/N > or = 3) and 8-100 microg/kg (S/N > or = 10), 
      respectively. The average recoveries of the eight compounds were 83.48%-96.97% in 
      milk and 82.70%-95.14% in milk powder. The relative standard deviations (RSDs) in 
      milk and milk powder were 3.86%-10.87% and 3.02%-9.81%, respectively. In 
      conclusion, the established method is convenient, accurate and sensitive so that 
      it can be applied to the determination of penicillin residues and enzymatic 
      metabolites in milk and milk powder.
FAU - Li, Wei
AU  - Li W
AD  - Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China. 
      liwei.305@163.com
FAU - Ai, Lianfeng
AU  - Ai L
AD  - Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China.
FAU - Guo, Chunhai
AU  - Guo C
AD  - Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China.
FAU - Ma, Yusong
AU  - Ma Y
AD  - Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China.
FAU - Dou, Caiyun
AU  - Dou C
AD  - Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Penicillins)
RN  - 0 (Powders)
RN  - 804826J2HU (Amoxicillin)
RN  - F93JSF8762 (penilloic acid)
RN  - Q42T66VG0C (Penicillin G)
SB  - IM
MH  - Amoxicillin
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Drug Residues/*analysis
MH  - Food Contamination/*analysis
MH  - Mass Spectrometry
MH  - Milk/*chemistry
MH  - Penicillin G/analogs & derivatives
MH  - Penicillins/*analysis
MH  - Powders
MH  - Solid Phase Extraction
MH  - *Tandem Mass Spectrometry
EDAT- 2014/01/18 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/01/18 06:00
PHST- 2014/01/18 06:00 [entrez]
PHST- 2014/01/18 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.3724/sp.j.1123.2013.03016 [doi]
PST - ppublish
SO  - Se Pu. 2013 Oct;31(10):946-53. doi: 10.3724/sp.j.1123.2013.03016.