PMID- 24432776 OWN - NLM STAT- MEDLINE DCOM- 20141118 LR - 20140117 IS - 1557-8070 (Electronic) IS - 1557-8070 (Linking) VI - 14 IP - 1 DP - 2014 Jan TI - Implementing planetary protection measures on the Mars Science Laboratory. PG - 27-32 LID - 10.1089/ast.2013.0989 [doi] AB - The Mars Science Laboratory (MSL), comprising a cruise stage; an aeroshell; an entry, descent, and landing system; and the radioisotope thermoelectric generator-powered Curiosity rover, made history with its unprecedented sky crane landing on Mars on August 6, 2012. The mission's primary science objective has been to explore the area surrounding Gale Crater and assess its habitability for past life. Because microbial contamination could profoundly impact the integrity of the mission and compliance with international treaty was required, planetary protection measures were implemented on MSL hardware to verify that bioburden levels complied with NASA regulations. By applying the proper antimicrobial countermeasures throughout all phases of assembly, the total bacterial endospore burden of MSL at the time of launch was kept to 2.78x10(5) spores, well within the required specification of less than 5.0x10(5) spores. The total spore burden of the exposed surfaces of the landed MSL hardware was 5.64x10(4), well below the allowed limit of 3.0x10(5) spores. At the time of launch, the MSL spacecraft was burdened with an average of 22 spores/m(2), which included both planned landed and planned impacted hardware. Here, we report the results of a campaign to implement and verify planetary protection measures on the MSL flight system. FAU - Benardini, James N 3rd AU - Benardini JN 3rd AD - Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology , Pasadena, California. FAU - La Duc, Myron T AU - La Duc MT FAU - Beaudet, Robert A AU - Beaudet RA FAU - Koukol, Robert AU - Koukol R LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PL - United States TA - Astrobiology JT - Astrobiology JID - 101088083 SB - IM MH - Bacteria/isolation & purification MH - *Containment of Biohazards MH - Equipment Contamination MH - *Extraterrestrial Environment MH - *Laboratories MH - *Mars MH - *Spacecraft EDAT- 2014/01/18 06:00 MHDA- 2014/11/19 06:00 CRDT- 2014/01/18 06:00 PHST- 2014/01/18 06:00 [entrez] PHST- 2014/01/18 06:00 [pubmed] PHST- 2014/11/19 06:00 [medline] AID - 10.1089/ast.2013.0989 [doi] PST - ppublish SO - Astrobiology. 2014 Jan;14(1):27-32. doi: 10.1089/ast.2013.0989.