PMID- 24433696
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20140117
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 49
IP  - 10
DP  - 2013 Oct
TI  - [Evaluation of calcium dobesilate for its anti-cataract potential in experimental 
      rat models].
PG  - 927-33
AB  - OBJECTIVE: To investigate the preventive and therapeutic effects of agent calcium 
      dobesilate(CDO) with different doses on the galactose cataract of rats. METHODS: 
      We chose fifty Wistar rats at 20- day old. Then, they were divided into 3 groups 
      at random. Choose 10 rats as the control group and gave normal diet; 10 rats as 
      the model group and fed with Gal solution ( drink 12.5% Gal solution from 1 to 7 
      days and 10%Gal solution from 8 to 21 days except for normal diet ) ; 30 rats as 
      the treatment group and fed with the same Gal solution as the model group, 
      besides they were divided into high dosage group, medium dosage group and low 
      dosage group equally and gave 300 mgxkg(-1)xd(-1), 150 mgxkg(-1)xd(-1), 75 
      mgxkg(-1)xd(-1) dose of calcium dobesilate respectively from the first day to the 
      end of experiment. The experiment lasts 21 days. Lens opacity were observed and 
      recorded by slit-lamp examination regularly. Activity of superoxide dismutase 
      (SOD) and glutathione peroxidase (GSH-px) and content of malondialdehyde (MDA) 
      were determined to estimate the effect of CDO . Lens fibers changes and 
      Histological changes were observed by scanning electron microscope (SEM) and 
      light microscope (LM) separately. The apoptosis rate of lens epithelium were 
      determined by TUNEL assay. RESULTS: The appearance of Lens opacity in model group 
      was more quickly than that in treatment group in model group, 3 eyes in degree 
      IV, 7 eyes in degree V, while in treatment group, 5 eyes in degree III, 3 eyes in 
      degree IV, 2 eyes in degree V (H = 7.12, P < 0.05). The activity of SOD and 
      GSH-px in treatment group is higher than mode group, but lower than control group 
      on 8th day, there was difference noticed in the activity of SOD (50.01 +/- 1.19), 
      (39.39 +/- 1.70) , treatment group (46.57 +/- 1.09, 46.42 +/- 0.87, 45.70 +/- 1.46) 
      U/mgProt (F = 88.70, P < 0.05) and the activity of GSH-px (42.92 +/- 0.97) , (12.70 
      +/- 1.17) , treatment group (29.16 +/- 1.05, 29.08 +/- 0.98, 28.25 +/- 0.98) nmol/mgprot 
      (F = 1071.89, P < 0.05) ]in 3 groups. The content of MDA in model group (3.43 +/- 
      0.15)nmol/mgprot is higher than treatment group (2.89 +/- 0.11, 2.99 +/- 0.12, 2.99 +/- 
      0.09)nmol/mgprot (F = 64.62; P < 0.05). There were no statistic significant 
      differences among high dosage group, medium dosage group and low dosage group . 
      The texture of lens fibres detected by SEM in the rats of model was more disorder 
      than treatment group. After HE staining, Lens epithelial cell detected by LM in 
      control group have a clear structure, however, Lens epithelial cell both in model 
      group and treatment group have changed from the initial single layer to 
      multi-storey. Junction between lenses fibers became decreased even disappeared . 
      The apoptosis rate of lens epithelium in treatment group[(2.37 +/- 0.17)%, (2.46 +/- 
      0.26)%, (2.79 +/- 0.41)%] is higher than control group (0.23 +/- 0.07) %, but is much 
      fewer than model group (4.99 +/- 0.51) % (chi(2) = 40.41;P < 0.05). CONCLUSION: CDO 
      with different doses could protect lens of rats against galactose damage and 
      there were no significant differences among the different doses of groups.
FAU - Zheng, Guang-ying
AU  - Zheng GY
AD  - Department of Ophthalmology, the First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou 450052, China. Email: zzzgy@zzu.edu.cn.
FAU - Jiang, Yu
AU  - Jiang Y
AD  - Department of Ophthalmology, the First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou 450052, China.
FAU - Wang, Rui-na
AU  - Wang RN
AD  - Department of Ophthalmology, the First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou 450052, China.
FAU - Hao, Li-li
AU  - Hao LL
AD  - Department of Ophthalmology, the First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou 450052, China.
FAU - Xu, Dong
AU  - Xu D
AD  - Department of Ophthalmology, the First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou 450052, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
RN  - 5921X1560Q (Calcium Dobesilate)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Animals
MH  - Calcium Dobesilate/*pharmacology/therapeutic use
MH  - Cataract/chemically induced/drug therapy/*prevention & control
MH  - Galactose
MH  - Rats
MH  - Rats, Wistar
EDAT- 2014/01/18 06:00
MHDA- 2014/09/16 06:00
CRDT- 2014/01/18 06:00
PHST- 2014/01/18 06:00 [entrez]
PHST- 2014/01/18 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 2013 Oct;49(10):927-33.