PMID- 24434684
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20140211
IS  - 1421-9921 (Electronic)
IS  - 0014-312X (Linking)
VI  - 51
IP  - 3-4
DP  - 2013
TI  - Hemoadsorption of high-mobility group box chromosomal protein 1 using a column 
      for large animals.
PG  - 181-90
LID - 10.1159/000357563 [doi]
AB  - BACKGROUND: High-mobility group box chromosomal protein 1 (HMGB1) has recently 
      been identified as an important mediator of various kinds of acute and chronic 
      inflammation. A method for efficiently removing HMGB1 from the systemic 
      circulation could be a promising therapy for HMGB1-mediated inflammatory 
      diseases. MATERIALS AND METHODS: In this study, we produced a new adsorbent 
      material by chemically treating polystyrene fiber. We first determined whether 
      the adsorbent material efficiently adsorbed HMGB1 in vitro using a bovine HMGB1 
      solution and a plasma sample from a swine model of acute liver failure. We then 
      constructed a column by embedding fabric sheets of the newly developed fibers 
      into a cartridge and tested the ability of the column to reduce plasma HMGB1 
      levels during a 4-hour extracorporeal hemoperfusion in a swine model of acute 
      liver failure. RESULTS: The in vitro adsorption test of the new fiber showed high 
      performance for HMGB1 adsorption (96% adsorption in the bovine HMGB1 solution and 
      94% in the acute liver failure swine plasma, 2 h incubation at 37 degrees C; p < 0.05 vs. 
      incubation with no adsorbent). In the in vivo study, the ratio of the HMGB1 
      concentration at the outlet versus the inlet of the column was significantly 
      lower in swine hemoperfused with the newly developed column (53 and 61% at the 
      beginning and end of perfusion, respectively) than in those animals hemoperfused 
      with the control column (94 and 93% at the beginning and end of perfusion, 
      respectively; p < 0.05). Moreover, the normalized plasma level of HMGB1 was 
      significantly lower during perfusion with the new column than with the control 
      column (p < 0.05 at 1, 2, and 3 h after initiation of perfusion). CONCLUSION: 
      These data suggest that the newly developed column has the potential to 
      effectively adsorb HMGB1 during hemoperfusion in swine.
FAU - Nishiyama, R
AU  - Nishiyama R
AD  - Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
FAU - Shinoda, M
AU  - Shinoda M
FAU - Tanabe, M
AU  - Tanabe M
FAU - Oshima, G
AU  - Oshima G
FAU - Takano, K
AU  - Takano K
FAU - Miyasho, T
AU  - Miyasho T
FAU - Fuchimoto, Y
AU  - Fuchimoto Y
FAU - Yamada, S
AU  - Yamada S
FAU - Inoue, T
AU  - Inoue T
FAU - Shimada, K
AU  - Shimada K
FAU - Suda, K
AU  - Suda K
FAU - Tanaka, M
AU  - Tanaka M
FAU - Hayashida, T
AU  - Hayashida T
FAU - Yagi, H
AU  - Yagi H
FAU - Kitago, M
AU  - Kitago M
FAU - Obara, H
AU  - Obara H
FAU - Itano, O
AU  - Itano O
FAU - Takeuchi, H
AU  - Takeuchi H
FAU - Kawachi, S
AU  - Kawachi S
FAU - Maruyama, I
AU  - Maruyama I
FAU - Kitagawa, Y
AU  - Kitagawa Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140109
PL  - Switzerland
TA  - Eur Surg Res
JT  - European surgical research. Europaische chirurgische Forschung. Recherches 
      chirurgicales europeennes
JID - 0174752
RN  - 0 (HMGB1 Protein)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - HMGB1 Protein/*blood/isolation & purification
MH  - Hemoperfusion/*methods
MH  - Liver Failure, Acute/blood/therapy
MH  - Male
MH  - Swine
EDAT- 2014/01/18 06:00
MHDA- 2014/11/19 06:00
CRDT- 2014/01/18 06:00
PHST- 2013/07/30 00:00 [received]
PHST- 2013/11/22 00:00 [accepted]
PHST- 2014/01/18 06:00 [entrez]
PHST- 2014/01/18 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
AID - 000357563 [pii]
AID - 10.1159/000357563 [doi]
PST - ppublish
SO  - Eur Surg Res. 2013;51(3-4):181-90. doi: 10.1159/000357563. Epub 2014 Jan 9.