PMID- 24435103
OWN - NLM
STAT- MEDLINE
DCOM- 20141031
LR  - 20231213
IS  - 1423-0356 (Electronic)
IS  - 0025-7931 (Linking)
VI  - 87
IP  - 3
DP  - 2014
TI  - Decreased maturation of dendritic cells in the central airways of COPD patients 
      is associated with VEGF, TGF-beta and vascularity.
PG  - 234-42
LID - 10.1159/000356749 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) have a pivotal role in the onset and regulation 
      of innate and adaptive immune responses. Moreover, DCs can interact with 
      angiogenic modulators, resulting in modification of their biology and 
      participation in angiogenesis. OBJECTIVES: This study was designed to evaluate 
      the relationship between the density of DCs, vascularity and expression of 
      angiogenic factors [vascular endothelial growth factor (VEGF), transforming 
      growth factor (TGF)-beta and basic fibroblast growth factor (bFGF)] in the central 
      airways of chronic obstructive pulmonary disease (COPD) patients. METHODS: The 
      study included 20 patients with moderate/severe COPD and 8 healthy control 
      subjects. Bronchial biopsies were evaluated by immunohistochemistry. Specimens 
      were examined for CD83 and CD207 to mark mature and immature DCs, respectively, 
      for collagen IV to evaluate vascularity, and for VEGF, TGF-beta and bFGF. RESULTS: 
      Compared to controls, COPD patients had a significant reduction of CD83+ cells 
      and an increased CD207/CD83 ratio (p < 0.05). Vascularity, VEGF, TGF-beta and bFGF 
      were also significantly increased in COPD patients as compared to controls (p < 
      0.01). In COPD patients, CD83+ cells were inversely related to VEGF and TGF-beta 
      expression (p < 0.05). Moreover, the CD207/CD83 ratio was positively related to 
      VEGF, TGF-beta and vascularity (p < 0.05). Finally, CD207+ cells were inversely 
      related to FEV1 (p < 0.05). CONCLUSION: Our results show a reduced maturation of 
      DCs in COPD that was related to airway vascularity and angiogenic factors (VEGF 
      and TGF-beta). Additionally, immature DCs were significantly related to disease 
      severity. We propose that the interplay between airway vascular changes, on one 
      hand, and DCs maturation on the other, may play a key role in the pathogenetic 
      mechanisms of COPD.
CI  - (c) 2013 S. Karger AG, Basel.
FAU - Zanini, Andrea
AU  - Zanini A
AD  - Department of Clinical and Experimental Medicine, University of Insubria, Varese, 
      Italy.
FAU - Spanevello, Antonio
AU  - Spanevello A
FAU - Baraldo, Simonetta
AU  - Baraldo S
FAU - Majori, Maria
AU  - Majori M
FAU - Della Patrona, Sabrina
AU  - Della Patrona S
FAU - Gumiero, Federico
AU  - Gumiero F
FAU - Aiello, Marina
AU  - Aiello M
FAU - Olivieri, Dario
AU  - Olivieri D
FAU - Saetta, Marina
AU  - Saetta M
FAU - Chetta, Alfredo
AU  - Chetta A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140114
PL  - Switzerland
TA  - Respiration
JT  - Respiration; international review of thoracic diseases
JID - 0137356
RN  - 0 (Antigens, CD)
RN  - 0 (CD207 protein, human)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD/*metabolism
MH  - Bronchi/immunology/*metabolism
MH  - Case-Control Studies
MH  - Dendritic Cells/immunology/*metabolism
MH  - Female
MH  - Fibroblast Growth Factor 2/immunology/*metabolism
MH  - Humans
MH  - Immunoglobulins/*metabolism
MH  - Immunohistochemistry
MH  - Lectins, C-Type/*metabolism
MH  - Male
MH  - Mannose-Binding Lectins/*metabolism
MH  - Membrane Glycoproteins/*metabolism
MH  - Middle Aged
MH  - Neovascularization, Pathologic/metabolism
MH  - Pulmonary Disease, Chronic Obstructive/immunology/*metabolism
MH  - Severity of Illness Index
MH  - Transforming Growth Factor beta/immunology/*metabolism
MH  - Vascular Endothelial Growth Factor A/immunology/*metabolism
MH  - CD83 Antigen
EDAT- 2014/01/18 06:00
MHDA- 2014/11/02 06:00
CRDT- 2014/01/18 06:00
PHST- 2013/08/08 00:00 [received]
PHST- 2013/10/10 00:00 [accepted]
PHST- 2014/01/18 06:00 [entrez]
PHST- 2014/01/18 06:00 [pubmed]
PHST- 2014/11/02 06:00 [medline]
AID - 000356749 [pii]
AID - 10.1159/000356749 [doi]
PST - ppublish
SO  - Respiration. 2014;87(3):234-42. doi: 10.1159/000356749. Epub 2014 Jan 14.