PMID- 24436208
OWN - NLM
STAT- MEDLINE
DCOM- 20141031
LR  - 20191210
IS  - 1520-636X (Electronic)
IS  - 0899-0042 (Linking)
VI  - 26
IP  - 2
DP  - 2014 Feb
TI  - HPLC-PDA-ORD bioassay of S-(+) and R-(-) clopidogrel on rat dried blood spots.
PG  - 102-7
LID - 10.1002/chir.22271 [doi]
AB  - A simple and rapid chiral high-performance liquid chromatography (HPLC) method 
      was developed and validated for bioanalysis of clopidogrel enantiomers on rat 
      dried blood spots (DBS). Clopidogrel enantiomers were extracted from DBS using 
      ethanol: methanol (80:20, v/v) and separated on a Chiralcel OJ-H column 
      containing cellulose tris (4-methly benzoate) as a polysaccharide stationary 
      phase using n-hexane-ethanol-diethylamine (70:30, 0.1 v/v) as a mobile phase at a 
      flow rate of 1.0 mL/min. The detection was carried out at 220 nm using a 
      photodiode array (PDA) detector while the elution order of the enantiomers was 
      determined by a polarimeter connected to PDA in series. The effect of hematocrit 
      on extraction of clopidogrel enantiomers from DBS was evaluated and no 
      interference from endogenous substances was noticed. The overall accuracy of (R) 
      and (S) enantiomers of clopidogrel from DBS were 91.6 and 89.2%, respectively. 
      The calibration curves were linear over the concentration range of 1-500 microg/mL 
      for both enantiomers. The results show that the method is specific, precise, and 
      reproducible (intra- and interday precision relative standard deviations (RSDs) 
      <10.0%). The stability of racemic clopidogrel was performed under all storage 
      conditions and the results were found to be well within the acceptance limits.
CI  - Copyright (c) 2014 Wiley Periodicals, Inc.
FAU - Rao, Ramisetti Nageswara
AU  - Rao RN
AD  - Analytical Chemistry Division, Discovery Laboratory, Indian Institute of Chemical 
      Technology, Tarnaka, Hyderabad, India.
FAU - Prasad, Katuri Guru
AU  - Prasad KG
FAU - Bindu Priya, Pullakandam
AU  - Bindu Priya P
FAU - Bijarji, Shriharsh
AU  - Bijarji S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20140117
PL  - United States
TA  - Chirality
JT  - Chirality
JID - 8914261
RN  - A74586SNO7 (Clopidogrel)
RN  - OM90ZUW7M1 (Ticlopidine)
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Blood Chemical Analysis/*methods
MH  - *Chromatography, High Pressure Liquid
MH  - Clopidogrel
MH  - Molecular Structure
MH  - Rats
MH  - Reproducibility of Results
MH  - Stereoisomerism
MH  - Ticlopidine/*analogs & derivatives/analysis/chemistry
MH  - Time Factors
OTO - NOTNLM
OT  - antiplatelets
OT  - cellulose tris (4-methly benzoate) on silica
OT  - clopidogrel
OT  - dried blood spots
OT  - enantiomers
OT  - hematocrit
EDAT- 2014/01/18 06:00
MHDA- 2014/11/02 06:00
CRDT- 2014/01/18 06:00
PHST- 2013/06/06 00:00 [received]
PHST- 2013/10/15 00:00 [accepted]
PHST- 2014/01/18 06:00 [entrez]
PHST- 2014/01/18 06:00 [pubmed]
PHST- 2014/11/02 06:00 [medline]
AID - 10.1002/chir.22271 [doi]
PST - ppublish
SO  - Chirality. 2014 Feb;26(2):102-7. doi: 10.1002/chir.22271. Epub 2014 Jan 17.