PMID- 24436278
OWN - NLM
STAT- MEDLINE
DCOM- 20140304
LR  - 20151119
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 141
IP  - 2
DP  - 2014 Feb
TI  - Double minutes and MYC amplification: a combined May-Grunwald Giemsa and 
      fluorescence in situ hybridization study.
PG  - 280-4
LID - 10.1309/AJCPWUBGT7C0LHIN [doi]
AB  - OBJECTIVES: To report the demonstration of double minutes with MYC amplification 
      in a case of myeloproliferative neoplasm with monocytosis in transformation by a 
      combination of standard karyotyping and interphase and metaphase fluorescence in 
      situ hybridization (FISH). METHODS: To determine the lineage involvement, we 
      applied combined morphology and an interphase FISH study using dual-color 
      break-apart probes for MYC on peripheral blood film. RESULTS: MYC amplification 
      was demonstrated in both myeloid and monocytic cells but not lymphocytes. The MYC 
      amplification was not associated with loss of MYC signals at the homologous 8q24 
      regions where the genes were located. Furthermore, the extent of MYC 
      amplification has been shown to diminish as the granulocytes mature. CONCLUSIONS: 
      Combined morphology and FISH study has shown a pluripotent myeloid disorder and 
      also an inverse relationship between cell maturity and MYC amplification.
FAU - Wong, K F
AU  - Wong KF
AD  - Dept of Pathology, Queen Elizabeth Hospital, 30 Gascoigne Rd, Kowloon, Hong Kong 
      SAR, China; kfwong@ha.org.hk.
FAU - Siu, Lisa L P
AU  - Siu LL
FAU - Wong, W S
AU  - Wong WS
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Chromatin)
RN  - 0 (Coloring Agents)
RN  - 0 (MYC protein, human)
RN  - 0 (May-Grunwald Giemsa)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - T42P99266K (Methylene Blue)
RN  - TDQ283MPCW (Eosine Yellowish-(YS))
SB  - IM
MH  - Aged, 80 and over
MH  - Chromatin/*genetics/pathology
MH  - Coloring Agents
MH  - Eosine Yellowish-(YS)
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelomonocytic, Chronic/*genetics/pathology
MH  - Methylene Blue
MH  - Proto-Oncogene Proteins c-myc/*genetics
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Genetics
OT  - Hematopathology
EDAT- 2014/01/18 06:00
MHDA- 2014/03/05 06:00
CRDT- 2014/01/18 06:00
PHST- 2014/01/18 06:00 [entrez]
PHST- 2014/01/18 06:00 [pubmed]
PHST- 2014/03/05 06:00 [medline]
AID - 141/2/280 [pii]
AID - 10.1309/AJCPWUBGT7C0LHIN [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2014 Feb;141(2):280-4. doi: 10.1309/AJCPWUBGT7C0LHIN.