PMID- 24438624
OWN - NLM
STAT- MEDLINE
DCOM- 20150128
LR  - 20181203
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 127
IP  - 2
DP  - 2014
TI  - Human adipose-derived mesenchymal stem cells: a better cell source for nervous 
      system regeneration.
PG  - 329-37
AB  - BACKGROUND: In order to suggest an ideal source of adult stem cells for the 
      treatment of nervous system diseases, MSCs from human adipose tissue and bone 
      marrow were isolated and studied to explore the differences with regard to cell 
      morphology, surface markers, neuronal differentiation capacity, especially the 
      synapse structure formation and the secretion of neurotrophic factors. METHODS: 
      The neuronal differentiation capacity of human mesenchymal stem cells from 
      adipose tissue (hADSCs) and bone marrow (hBMSCs) was determined based on nissl 
      body and synapse structure formation, and neural factor secretion function. 
      hADSCs and hBMSCs were isolated and differentiated into neuron-like cells with 
      rat brain-conditioned medium, a potentially rich source of neuronal 
      differentiation promoting signals. Specific neuronal proteins and neural factors 
      were detected by immunohistochemistry and enzyme-linked immunosorbent assay 
      analysis, respectively. RESULTS: Flow cytometric analysis showed that both cell 
      types had similar phenotypes. Cell growth curves showed that hADSCs proliferated 
      more quickly than hBMSCs. Both kinds of cells were capable of osteogenic and 
      adipogenic differentiation. The morphology of hADSCs and hBMSCs changed during 
      neuronal differentiation and displayed neuron-like cell appearance after 14 days' 
      differentiation. Both hADSCs and hBMSCs were able to differentiate into 
      neuron-like cells based on their production of neuron specific proteins including 
      beta-tubulin-III, neuron-specific enolase (NSE), nissl bodies, and their ability to 
      secrete brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF). 
      Assessment of synaptop hysin and growth-associated protein-43 (GAP-43) suggested 
      synapse structure formation in differentiated hADSCs and hBMSCs. CONCLUSIONS: Our 
      results demonstrate that hADSCs have neuronal differentiation potential similar 
      to hBMSC, but with a higher proliferation capacity than hBMSC. Adipose tissue is 
      abundant, easily available and would be a potential ideal source of adult stem 
      cells for neural-related clinical research and application.
FAU - Han, Chao
AU  - Han C
AD  - Regenerative Medicine Center, the First Affiliated Hospital of Dalian Medical 
      University, Dalian, Liaoning 116011, China.
FAU - Zhang, Liang
AU  - Zhang L
AD  - Department of Orthopedics, Beijing Friendship Hospital, Capital Medical 
      University, Beijing 100050, China.
FAU - Song, Lin
AU  - Song L
AD  - Regenerative Medicine Center, the First Affiliated Hospital of Dalian Medical 
      University, Dalian, Liaoning 116011, China; School of Life Science and 
      Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Regenerative Medicine Center, the First Affiliated Hospital of Dalian Medical 
      University, Dalian, Liaoning 116011, China.
FAU - Zou, Wei
AU  - Zou W
AD  - College of Life Science, Liaoning Normal University, Dalian, Liaoning 116011, 
      China.
FAU - Piao, Hua
AU  - Piao H
AD  - College of Basic Medical Science, Dalian Medical University, Dalian, Liaoning 
      116027, China.
FAU - Liu, Jing
AU  - Liu J
AD  - Regenerative Medicine Center, the First Affiliated Hospital of Dalian Medical 
      University, Dalian, Liaoning 116011, China; Institute of Integrative Medicine, 
      Dalian Medical University, Dalian, Liaoning 116027, China. Email: 
      liujing.dlrmc@hotmail. com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Adult
MH  - Bone Marrow Cells/cytology
MH  - Cell Differentiation/*physiology
MH  - Cells, Cultured
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Middle Aged
MH  - Nerve Regeneration/physiology
MH  - Neurons/*cytology
MH  - Young Adult
EDAT- 2014/01/21 06:00
MHDA- 2015/01/30 06:00
CRDT- 2014/01/21 06:00
PHST- 2014/01/21 06:00 [entrez]
PHST- 2014/01/21 06:00 [pubmed]
PHST- 2015/01/30 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2014;127(2):329-37.