PMID- 24438897 OWN - NLM STAT- MEDLINE DCOM- 20140829 LR - 20181203 IS - 1477-2566 (Electronic) IS - 1465-3249 (Linking) VI - 16 IP - 2 DP - 2014 Feb TI - Microencapsulated VEGF gene-modified umbilical cord mesenchymal stromal cells promote the vascularization of tissue-engineered dermis: an experimental study. PG - 160-9 LID - S1465-3249(13)00771-8 [pii] LID - 10.1016/j.jcyt.2013.10.014 [doi] AB - BACKGROUND AIMS: Tissue-engineered dermis (TED) is thought to be the best treatment for skin defect wounds; however, lack of vascular structures in these products can cause slow vascularization or even transplant failure. We assessed the therapeutic potential of microencapsulated human umbilical cord mesenchymal stromal cells (hUCMSCs) expressing vascular endothelial growth factor (VEGF) in vascularization of TED. METHODS: hUCMSCs were isolated by means of enzymatic digestion and identified by means of testing biological characteristics. hUCMSCs were induced to differentiate into dermal fibroblasts in conditioned induction media. Collagen-chitosan laser drilling acellular dermal matrix (ADM) composite scaffold was prepared by means of the freeze dehydration and dehydrothermal cross-linking method. hUCMSC-derived fibroblasts were implanted on composite scaffolds to construct TED. TED with microencapsulated VEGF gene-modified hUCMSCs was then transplanted into skin defect wounds in pigs. The angiogenesis of TED at 1 week and status of wound healing at 3 weeks were observed. RESULTS: The collagen-chitosan laser ADM composite has a uniform microporous structure. This composite has been used to grow hUCMSC-derived fibroblasts in vitro and to successfully construct stem cell-derived TED. Microencapsulated VEGF gene-modified hUCMSCs were prepared with the use of a sodium alginate-barium chloride one-step encapsulation technology. Seven days after the transplantation of the stem cell-derived TED and microencapsulated VEGF gene-modified hUCMSCs into the skin defect wounds on the backs of miniature pigs, the VEGF expression increased and the TED had a higher degree of vascularization. Re-epithelialization of the wound was completed after 3 weeks. CONCLUSIONS: Microencapsulated VEGF gene-modified hUCMSCs can effectively improve the vascularization of TED and consequently the quality of wound healing. CI - Copyright (c) 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. FAU - Han, Yanfu AU - Han Y AD - Department of Plastic Surgery, Affiliated Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China; Department of Burn and Plastic Surgery, Burns Institute, The First Affiliated Hospital of PLA General Hospital, Beijing, People's Republic of China. FAU - Tao, Ran AU - Tao R AD - Department of Plastic Surgery, PLA General Hospital, Beijing, People's Republic of China. FAU - Han, Yanqing AU - Han Y AD - School of Electrical and Information Engineering, Wuhan Institute of Technology, Wuhan, People's Republic of China. FAU - Sun, Tianjun AU - Sun T AD - Department of Burn and Plastic Surgery, Burns Institute, The First Affiliated Hospital of PLA General Hospital, Beijing, People's Republic of China. FAU - Chai, Jiake AU - Chai J AD - Department of Burn and Plastic Surgery, Burns Institute, The First Affiliated Hospital of PLA General Hospital, Beijing, People's Republic of China. Electronic address: cjk304@126.com. FAU - Xu, Guang AU - Xu G AD - Department of Plastic Surgery, Affiliated Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China. FAU - Liu, Jing AU - Liu J AD - Department of Plastic Surgery, Affiliated Beijing Shijitan Hospital, Capital Medical University, Beijing, People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Cytotherapy JT - Cytotherapy JID - 100895309 RN - 0 (Capsules) RN - 0 (Vascular Endothelial Growth Factor A) RN - 9007-34-5 (Collagen) RN - 9012-76-4 (Chitosan) SB - IM MH - Animals MH - Capsules/*metabolism MH - Cells, Cultured MH - Chitosan/metabolism MH - Collagen/metabolism MH - Dermis/blood supply/injuries/*physiology MH - Extracellular Matrix/metabolism MH - Fibroblasts/*physiology MH - Gene Transfer Techniques MH - Guided Tissue Regeneration MH - Heterografts MH - Humans MH - Induced Pluripotent Stem Cells/*physiology MH - Mesenchymal Stem Cells/metabolism/*physiology MH - Models, Animal MH - Neovascularization, Physiologic MH - Skin Diseases/*therapy MH - *Skin Transplantation MH - Swine MH - Swine, Miniature MH - Tissue Engineering MH - Tissue Scaffolds/statistics & numerical data MH - Transgenes/genetics MH - Umbilical Cord/cytology MH - Vascular Endothelial Growth Factor A/genetics/*metabolism MH - Wound Healing OTO - NOTNLM OT - microencapsulation OT - tissue-engineered dermis OT - umbilical cord mesenchymal stromal cells OT - vascular endothelial growth factor OT - vascularization OT - wound healing EDAT- 2014/01/21 06:00 MHDA- 2014/08/30 06:00 CRDT- 2014/01/21 06:00 PHST- 2013/05/29 00:00 [received] PHST- 2013/10/25 00:00 [revised] PHST- 2013/10/28 00:00 [accepted] PHST- 2014/01/21 06:00 [entrez] PHST- 2014/01/21 06:00 [pubmed] PHST- 2014/08/30 06:00 [medline] AID - S1465-3249(13)00771-8 [pii] AID - 10.1016/j.jcyt.2013.10.014 [doi] PST - ppublish SO - Cytotherapy. 2014 Feb;16(2):160-9. doi: 10.1016/j.jcyt.2013.10.014.