PMID- 24439291
OWN - NLM
STAT- MEDLINE
DCOM- 20140904
LR  - 20220129
IS  - 0091-679X (Print)
IS  - 0091-679X (Linking)
VI  - 119
DP  - 2014
TI  - The use of microarrays and fluorescence in situ hybridization for the study of 
      mechanotransduction from topography.
PG  - 293-309
LID - B978-0-12-416742-1.00015-9 [pii]
LID - 10.1016/B978-0-12-416742-1.00015-9 [doi]
AB  - The combination of transcriptomic analysis and fluorescence in situ hybridization 
      (FISH) provides a robust methodology to study genomic changes in different 
      biological conditions. Microarrays allow a global study of gene expression in 
      response to the conditions of interest, with comparison between control(s) and 
      one or more test condition(s). The messenger RNA amplification step permits 
      detection of even low abundance transcripts, a critical advantage for 
      applications such as biomaterials research, where the starting material may be 
      limited. Different types of microarrays are commercially available that allow the 
      investigation of specific features, such as exon arrays, microRNA arrays, and 
      gene arrays. Microarrays are available for different model organisms, but we use 
      Affymetrix (R) HuGene (R) ST (Sense Target) arrays, a type of gene array for analysis 
      of human samples. FISH involves fluorescent detection of probe DNA hybridized to 
      an in situ chromosomal target that can be either whole chromosomes or chromosomal 
      segments. The overall hybridization is similar to labeling with radioactive 
      probes but the incorporation of fluorescent detection of the probe sequences 
      allows for high sensitivity in a simple and quick assay. FISH can be applied to a 
      variety of specimen types depending on the study of interest. In this chapter, we 
      describe the methodologies of these two techniques and provide technical tips 
      that should help overcome challenges in carrying them out.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - McNamara, Laura E
AU  - McNamara LE
AD  - Centre for Cell Engineering, Institute of Medical, Veterinary and Life Sciences, 
      University of Glasgow, Glasgow, United Kingdom.
FAU - Dalby, Matthew J
AU  - Dalby MJ
AD  - Centre for Cell Engineering, Institute of Medical, Veterinary and Life Sciences, 
      University of Glasgow, Glasgow, United Kingdom.
FAU - Tsimbouri, Monica P
AU  - Tsimbouri MP
AD  - Centre for Cell Engineering, Institute of Medical, Veterinary and Life Sciences, 
      University of Glasgow, Glasgow, United Kingdom.
LA  - eng
GR  - BB/G008868/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - JF20604/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Cell Biol
JT  - Methods in cell biology
JID - 0373334
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosomes/genetics
MH  - DNA Probes/chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Mechanotransduction, Cellular
MH  - Microarray Analysis/*methods
OTO - NOTNLM
OT  - Chromosome
OT  - Fluorescence in situ hybridization
OT  - Gene expression
OT  - Microarrays
OT  - Microscopy
OT  - RNA
EDAT- 2014/01/21 06:00
MHDA- 2014/09/05 06:00
CRDT- 2014/01/21 06:00
PHST- 2014/01/21 06:00 [entrez]
PHST- 2014/01/21 06:00 [pubmed]
PHST- 2014/09/05 06:00 [medline]
AID - B978-0-12-416742-1.00015-9 [pii]
AID - 10.1016/B978-0-12-416742-1.00015-9 [doi]
PST - ppublish
SO  - Methods Cell Biol. 2014;119:293-309. doi: 10.1016/B978-0-12-416742-1.00015-9.