PMID- 24439447
OWN - NLM
STAT- MEDLINE
DCOM- 20141103
LR  - 20191210
IS  - 1879-0070 (Electronic)
IS  - 0732-8893 (Linking)
VI  - 78
IP  - 4
DP  - 2014 Apr
TI  - Rapid detection of Enterococcus spp. direct from blood culture bottles using 
      Enterococcus QuickFISH method: a multicenter investigation.
PG  - 338-42
LID - S0732-8893(13)00646-9 [pii]
LID - 10.1016/j.diagmicrobio.2013.12.004 [doi]
AB  - The performance of a diagnostic method for detection and identification of 
      Enterococcus spp. directly from positive blood culture was evaluated in a 
      clinical study. The method, Enterococcus QuickFISH BC, is a second-generation 
      peptide nucleic acid (PNA) fluorescence in situ hybridization (FISH) test, which 
      uses a simplified, faster assay procedure. The test uses fluorescently labeled 
      PNA probes targeting 16S rRNA to differentiate Enterococcus faecalis from other 
      Enterococcus spp. by the color of the cellular fluorescence. Three hundred 
      fifty-six routine blood culture samples were tested; only 2 discordant results 
      were recorded. The sensitivities for detection of Enterococcus faecalis and 
      non-faecalis Enterococcus were 100% (106/106) and 97.0% (65/67), respectively, 
      and the combined specificity of the assay was 100%. The combined positive and 
      negative predictive values of the assay were 100% (171/171) and 98.9% (185/187), 
      respectively.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Deck, Melissa K
AU  - Deck MK
AD  - AdvanDx Inc., Woburn, MA. Electronic address: mkd@advandx.com.
FAU - Anderson, Erica S
AU  - Anderson ES
AD  - AdvanDx Inc., Woburn, MA.
FAU - Buckner, Rebecca J
AU  - Buckner RJ
AD  - Indiana University School of Medicine, Indianapolis, IN.
FAU - Colasante, Georgia
AU  - Colasante G
AD  - Health Network Laboratories, Allentown, PA.
FAU - Davis, Thomas E
AU  - Davis TE
AD  - Indiana University School of Medicine, Indianapolis, IN.
FAU - Coull, James M
AU  - Coull JM
AD  - AdvanDx Inc., Woburn, MA.
FAU - Crystal, Benjamin
AU  - Crystal B
AD  - AdvanDx Inc., Woburn, MA.
FAU - Latta, Phyllis Della
AU  - Latta PD
AD  - Columbia University Medical Center, NewYork-Presbyterian Medical Center, New 
      York, NY.
FAU - Fuchs, Martin
AU  - Fuchs M
AD  - AdvanDx Inc., Woburn, MA.
FAU - Fuller, Deanna
AU  - Fuller D
AD  - Indiana University School of Medicine, Indianapolis, IN.
FAU - Harris, Will
AU  - Harris W
AD  - AdvanDx Inc., Woburn, MA.
FAU - Hazen, Kevin
AU  - Hazen K
AD  - Duke University, Durham, NC.
FAU - Klimas, Lisa L
AU  - Klimas LL
AD  - AdvanDx Inc., Woburn, MA.
FAU - Lindao, Daniel
AU  - Lindao D
AD  - Health Network Laboratories, Allentown, PA.
FAU - Meltzer, Michelle C
AU  - Meltzer MC
AD  - AdvanDx Inc., Woburn, MA.
FAU - Morgan, Margie
AU  - Morgan M
AD  - Cedars-Sinai Medical Center, Los Angeles, CA.
FAU - Shepard, Janeen
AU  - Shepard J
AD  - AdvanDx Inc., Woburn, MA.
FAU - Stevens, Sharon
AU  - Stevens S
AD  - AdvanDx Inc., Woburn, MA.
FAU - Wu, Fann
AU  - Wu F
AD  - Columbia University Medical Center, NewYork-Presbyterian Medical Center, New 
      York, NY.
FAU - Fiandaca, Mark J
AU  - Fiandaca MJ
AD  - AdvanDx Inc., Woburn, MA.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Multicenter Study
DEP - 20131214
PL  - United States
TA  - Diagn Microbiol Infect Dis
JT  - Diagnostic microbiology and infectious disease
JID - 8305899
SB  - IM
MH  - Blood/*microbiology
MH  - Enterococcus/*classification/genetics/*isolation & purification
MH  - Gram-Positive Bacterial Infections/*diagnosis/microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Diagnostic Techniques/*methods
MH  - Sensitivity and Specificity
MH  - Sepsis/*diagnosis/microbiology
OTO - NOTNLM
OT  - Enterococcus
OT  - PNA
OT  - QuickFISH
OT  - septicemia
EDAT- 2014/01/21 06:00
MHDA- 2014/11/05 06:00
CRDT- 2014/01/21 06:00
PHST- 2013/08/08 00:00 [received]
PHST- 2013/12/03 00:00 [revised]
PHST- 2013/12/08 00:00 [accepted]
PHST- 2014/01/21 06:00 [entrez]
PHST- 2014/01/21 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
AID - S0732-8893(13)00646-9 [pii]
AID - 10.1016/j.diagmicrobio.2013.12.004 [doi]
PST - ppublish
SO  - Diagn Microbiol Infect Dis. 2014 Apr;78(4):338-42. doi: 
      10.1016/j.diagmicrobio.2013.12.004. Epub 2013 Dec 14.