PMID- 24441193
OWN - NLM
STAT- MEDLINE
DCOM- 20141022
LR  - 20211203
IS  - 2038-8306 (Electronic)
IS  - 1121-760X (Print)
IS  - 1121-760X (Linking)
VI  - 57
IP  - 4
DP  - 2013 Dec 4
TI  - Localization of alphanubeta6 integrin-TGF-beta1/Smad3, mTOR and PPARgamma in experimental 
      colorectal fibrosis.
PG  - e40
LID - 10.4081/ejh.2013.e40 [doi]
LID - e40
AB  - A simultaneous action of several pro-fibrotic mediators appears relevant in the 
      development of fibrosis. There are evidences that transforming growth factor-beta 
      (TGF-beta)/Smad3 pathway forms with alphavbeta6 integrin, mammalian target of Rapamycin 
      (mTOR) and peroxisome proliferator-activated receptor-gamma (PPARgamma) a complex 
      signalling network with extensive crosstalk and strong effects on fibrosis 
      development. The present study evaluated the expression of TGFbeta, Smad3, alphavbeta6 
      integrin, mTOR and PPARgamma in 2,4,6-trinitrobenzenesulphonic acid (TNBS)-induced 
      colorectal fibrosis in Smad3 wild-type (WT) and null mice. Smad3 WT mice treated 
      with TNBS developed a marked colorectal fibrosis and showed a concomitant 
      up-regulation of TGFbeta, Smad3, alphavbeta6 and mTOR and a reduction of PPARgamma expression. 
      On the other hand, Smad3 Null mice similarly treated with TNBS did not develop 
      fibrosis and showed a very low or even absent expression of TGFbeta, Smad3, alphavbeta6 and 
      mTOR and a marked over-expression of PPARgamma. At the same time the expression of 
      alpha-smooth muscle actin (a marker of activated myofibroblasts), collagen I-III and 
      connective tissue growth factor (a downstream effector of TGFbeta/Smad3-induced 
      extracellular matrix proteins) were up-regulated in Smad3 WT mice treated with 
      TNBS compared to Null TNBS-treated mice. These preliminary results suggest a 
      possible interaction between these pro-fibrotic molecules in the development of 
      intestinal fibrosis.
FAU - Latella, G
AU  - Latella G
AD  - University of L'Aquila. giolatel@tin.it.
FAU - Vetuschi, A
AU  - Vetuschi A
FAU - Sferra, R
AU  - Sferra R
FAU - Speca, S
AU  - Speca S
FAU - Gaudio, E
AU  - Gaudio E
LA  - eng
PT  - Journal Article
DEP - 20131204
PL  - Italy
TA  - Eur J Histochem
JT  - European journal of histochemistry : EJH
JID - 9207930
RN  - 0 (Actins)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Integrins)
RN  - 0 (PPAR gamma)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (integrin alphavbeta6)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Antigens, Neoplasm/*metabolism
MH  - Colon/drug effects/*pathology
MH  - Fibrosis
MH  - Integrins/*metabolism
MH  - Mice
MH  - PPAR gamma/*metabolism
MH  - Signal Transduction
MH  - Smad3 Protein/genetics/*metabolism
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Transforming Growth Factor beta1/*metabolism
MH  - Trinitrobenzenesulfonic Acid
PMC - PMC3896042
EDAT- 2014/01/21 06:00
MHDA- 2014/10/23 06:00
PMCR- 2013/12/04
CRDT- 2014/01/21 06:00
PHST- 2013/07/09 00:00 [received]
PHST- 2013/11/04 00:00 [accepted]
PHST- 2013/11/26 00:00 [revised]
PHST- 2014/01/21 06:00 [entrez]
PHST- 2014/01/21 06:00 [pubmed]
PHST- 2014/10/23 06:00 [medline]
PHST- 2013/12/04 00:00 [pmc-release]
AID - ejh.2013.e40 [pii]
AID - 10.4081/ejh.2013.e40 [doi]
PST - epublish
SO  - Eur J Histochem. 2013 Dec 4;57(4):e40. doi: 10.4081/ejh.2013.e40.