PMID- 24442604
OWN - NLM
STAT- MEDLINE
DCOM- 20150204
LR  - 20211021
IS  - 1567-2387 (Electronic)
IS  - 1567-2379 (Linking)
VI  - 45
IP  - 4
DP  - 2014 Aug
TI  - N-Acetylcysteine counteracts oxidative stress and protects alveolar epithelial 
      cells from lung contusion-induced apoptosis in rats with blunt chest trauma.
PG  - 463-71
LID - 10.1007/s10735-014-9563-6 [doi]
AB  - The aim of this study was to investigate the protective effects of 
      N-acetylcysteine (NAC) on peroxidative and apoptotic changes in the contused 
      lungs of rats following blunt chest trauma. The rats were randomly divided into 
      three groups: control, contusion, and contusion + NAC. All the rats, apart from 
      those in the control group, performed moderate lung contusion. A daily 
      intramuscular NAC injection (150 mg/kg) was given immediately following the blunt 
      chest trauma and was continued for two additional days following cessation of the 
      trauma. Samples of lung tissue were taken in order to evaluate the tissue 
      malondialdehyde (MDA) level, histopathology, and epithelial cell apoptosis using 
      terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and 
      active caspase-3 immunostaining. In addition, we immunohistochemically evaluated 
      the expression of surfactant protein D (SP-D) in the lung tissue. The blunt chest 
      trauma-induced lung contusion resulted in severe histopathological injury, as 
      well as an increase in the MDA level and in the number of cells identified on 
      TUNEL assay together with active caspase-3 positive epithelial cells, but a 
      decrease in the number of SP-D positive alveolar type 2 (AT-2) cells. NAC 
      treatment effectively attenuated histopathologic, peroxidative, and apoptotic 
      changes, as well as reducing alterations in SP-D expression in the lung tissue. 
      These findings indicate that the beneficial effects of NAC administrated 
      following blunt chest trauma is related to the regulation of oxidative stress and 
      apoptosis.
FAU - Topcu-Tarladacalisir, Yeter
AU  - Topcu-Tarladacalisir Y
AD  - Department of Histology and Embryology, Faculty of Medicine, Trakya University, 
      Edirne, 22030, Turkey, yeter_topcu@yahoo.com.
FAU - Tarladacalisir, Taner
AU  - Tarladacalisir T
FAU - Sapmaz-Metin, Melike
AU  - Sapmaz-Metin M
FAU - Karamustafaoglu, Altemur
AU  - Karamustafaoglu A
FAU - Uz, Yesim Hulya
AU  - Uz YH
FAU - Akpolat, Meryem
AU  - Akpolat M
FAU - Cerkezkayabekir, Aysegul
AU  - Cerkezkayabekir A
FAU - Turan, Fatma Nesrin
AU  - Turan FN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140118
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*therapeutic use
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Contusions/*drug therapy
MH  - Epithelial Cells/*cytology/*drug effects
MH  - Female
MH  - Lung Injury/*drug therapy
MH  - Oxidative Stress/*drug effects
MH  - Pulmonary Alveoli/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Thoracic Injuries/*drug therapy
EDAT- 2014/01/21 06:00
MHDA- 2015/02/05 06:00
CRDT- 2014/01/21 06:00
PHST- 2013/10/28 00:00 [received]
PHST- 2014/01/09 00:00 [accepted]
PHST- 2014/01/21 06:00 [entrez]
PHST- 2014/01/21 06:00 [pubmed]
PHST- 2015/02/05 06:00 [medline]
AID - 10.1007/s10735-014-9563-6 [doi]
PST - ppublish
SO  - J Mol Histol. 2014 Aug;45(4):463-71. doi: 10.1007/s10735-014-9563-6. Epub 2014 
      Jan 18.