PMID- 24443176
OWN - NLM
STAT- MEDLINE
DCOM- 20150406
LR  - 20211021
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Print)
IS  - 0895-8696 (Linking)
VI  - 53
IP  - 4
DP  - 2014 Aug
TI  - Genomic organization and identification of promoter regions for the BDNF gene in 
      the pond turtle Trachemys scripta elegans.
PG  - 626-36
LID - 10.1007/s12031-014-0229-3 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is an important regulator of neuronal 
      development and synaptic function. The BDNF gene undergoes significant 
      activity-dependent regulation during learning. Here, we identified the BDNF 
      promoter regions, transcription start sites, and potential regulatory sequences 
      for BDNF exons I-III that may contribute to activity-dependent gene and protein 
      expression in the pond turtle Trachemys scripta elegans (tBDNF). By using 
      transfection of BDNF promoter/luciferase plasmid constructs into human 
      neuroblastoma SHSY5Y cells and mouse embryonic fibroblast NIH3T3 cells, we 
      identified the basal regulatory activity of promoter sequences located upstream 
      of each tBDNF exon, designated as pBDNFI-III. Further, through chromatin 
      immunoprecipitation (ChIP) assays, we detected CREB binding directly to exon I 
      and exon III promoters, while BHLHB2, but not CREB, binds within the exon II 
      promoter. Elucidation of the promoter regions and regulatory protein binding 
      sites in the tBDNF gene is essential for understanding the regulatory mechanisms 
      that control tBDNF gene expression.
FAU - Ambigapathy, Ganesh
AU  - Ambigapathy G
AD  - Neuroscience Group, Division of Basic Biomedical Sciences, University of South 
      Dakota Sanford School of Medicine, 414 E. Clark St., Vermillion, SD, 57010, USA, 
      ganesh.ambigapathy@usd.edu.
FAU - Zheng, Zhaoqing
AU  - Zheng Z
FAU - Keifer, Joyce
AU  - Keifer J
LA  - eng
GR  - R01 NS051187/NS/NINDS NIH HHS/United States
GR  - NS051187/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140118
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Reptilian Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Exons
MH  - *Genome
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - NIH 3T3 Cells
MH  - *Promoter Regions, Genetic
MH  - Reptilian Proteins/*genetics/metabolism
MH  - Turtles/*genetics
PMC - PMC4103967
MID - NIHMS585921
EDAT- 2014/01/21 06:00
MHDA- 2015/04/07 06:00
PMCR- 2014/08/01
CRDT- 2014/01/21 06:00
PHST- 2013/11/20 00:00 [received]
PHST- 2014/01/06 00:00 [accepted]
PHST- 2014/01/21 06:00 [entrez]
PHST- 2014/01/21 06:00 [pubmed]
PHST- 2015/04/07 06:00 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 10.1007/s12031-014-0229-3 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2014 Aug;53(4):626-36. doi: 10.1007/s12031-014-0229-3. Epub 2014 
      Jan 18.