PMID- 24443715
OWN - NLM
STAT- MEDLINE
DCOM- 20140512
LR  - 20220316
IS  - 1479-6805 (Electronic)
IS  - 0022-0795 (Linking)
VI  - 221
IP  - 1
DP  - 2014 Apr
TI  - Exogenous glucagon-like peptide 1 reduces contractions in human colon circular 
      muscle.
PG  - 29-37
LID - 10.1530/JOE-13-0525 [doi]
AB  - Glucagon-like peptide 1 (GLP1) is a naturally occurring peptide secreted by 
      intestinal L-cells. Though its primary function is to serve as an incretin, GLP1 
      reduces gastrointestinal motility. However, only a handful of animal studies have 
      specifically evaluated the influence of GLP1 on colonic motility. Consequently, 
      the aims of this study were to investigate the effects induced by exogenous GLP1, 
      to analyze the mechanism of action, and to verify the presence of GLP1 receptors 
      (GLP1Rs) in human colon circular muscular strips. Organ bath technique, RT-PCR, 
      western blotting, and immunofluorescence were used. In human colon, exogenous 
      GLP1 reduced, in a concentration-dependent manner, the amplitude of the 
      spontaneous contractions without affecting the frequency and the resting basal 
      tone. This inhibitory effect was significantly reduced by exendin (9-39), a GLP1R 
      antagonist, which per se significantly increased the spontaneous mechanical 
      activity. Moreover, it was abolished by tetrodotoxin, a neural blocker, or 
      Nomega-nitro-l-arginine - a blocker of neuronal nitric oxide synthase (nNOS). The 
      biomolecular analysis revealed a genic and protein expression of the GLP1R in the 
      human colon. The double-labeling experiments with anti-neurofilament or anti-nNOS 
      showed, for the first time, that immunoreactivity for the GLP1R was expressed in 
      nitrergic neurons of the myenteric plexus. In conclusion, the results of this 
      study suggest that GLP1R is expressed in the human colon and, once activated by 
      exogenous GLP1, mediates an inhibitory effect on large intestine motility through 
      NO neural release.
FAU - Amato, Antonella
AU  - Amato A
AD  - Laboratorio di Fisiologia Generale, Dipartimento di Scienze e Tecnologie 
      Biologiche Chimiche e Farmaceutiche (STEBICEF), Universita di Palermo, Viale 
      delle Scienze, 90128 Palermo, Italy Mediterranean Institute for Transplantation 
      and Advanced Specialized Therapies (ISMETT), Palermo, Italy.
FAU - Baldassano, Sara
AU  - Baldassano S
FAU - Liotta, Rosa
AU  - Liotta R
FAU - Serio, Rosa
AU  - Serio R
FAU - Mule, Flavia
AU  - Mule F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140307
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (GLP1R protein, human)
RN  - 0 (Glucagon-Like Peptide-1 Receptor)
RN  - 0 (Receptors, Glucagon)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Colon/*physiology
MH  - *Down-Regulation
MH  - Female
MH  - *Gastrointestinal Motility
MH  - Glucagon-Like Peptide 1/*metabolism
MH  - Glucagon-Like Peptide-1 Receptor
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Middle Aged
MH  - *Muscle Contraction
MH  - Muscles/*physiology
MH  - Receptors, Glucagon/genetics/metabolism
OTO - NOTNLM
OT  - GLP-1 receptor expression
OT  - colonic motility
OT  - intestinal peptides
OT  - nitric oxide
EDAT- 2014/01/21 06:00
MHDA- 2014/05/13 06:00
CRDT- 2014/01/21 06:00
PHST- 2014/01/21 06:00 [entrez]
PHST- 2014/01/21 06:00 [pubmed]
PHST- 2014/05/13 06:00 [medline]
AID - JOE-13-0525 [pii]
AID - 10.1530/JOE-13-0525 [doi]
PST - epublish
SO  - J Endocrinol. 2014 Mar 7;221(1):29-37. doi: 10.1530/JOE-13-0525. Print 2014 Apr.