PMID- 24444121 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20140124 LR - 20211021 IS - 1758-5996 (Print) IS - 1758-5996 (Electronic) IS - 1758-5996 (Linking) VI - 6 IP - 1 DP - 2014 Jan 21 TI - Measurement of the levels of leptin, BDNF associated with polymorphisms LEP G2548A, LEPR Gln223Arg and BDNF Val66Met in Thai with metabolic syndrome. PG - 6 LID - 10.1186/1758-5996-6-6 [doi] AB - BACKGROUND: Metabolic syndrome is a cluster of metabolic risk factors including dyslipidemia, impaired glucose tolerance, hypertension and central obesity. BDNF (Brain-derived neurotrophic factor) and leptin have been implied in the energy homeostasis. The purposes of this study were to examine concentrations of leptin, BDNF and biochemical parameters in metabolic-syndrome subjects and healthy controls, and also to search for associations of leptin gene (LEP) G2548A, leptin receptor gene (LEPR) Gln223Arg, and BDNF gene (BDNF) Val66Met polymorphisms with leptin levels, BDNF levels and metabolic syndrome among Thais. METHODS: The case-controlled design was performed using 322 Thai volunteers (160 metabolic-syndrome subjects; 162 controls) during the health screening program. Metabolic syndrome was assessed by using the modified National Cholesterol Education Program, Adult Treatment Panel III criteria. The levels of leptin, BDNF, insulin, glucose and lipids were measured in samples. Genotyping of LEP G2548A, LEPR Gln223Arg and BDNF Val66Met was carried out using polymerase chain reaction-restriction fragment length polymorphism technique. RESULTS: Serum leptin levels were significantly higher in the metabolic-syndrome group than the control group (p < 0.01), but the BDNF difference between them was not significant. Significant associations of LEPR Gln223Arg polymorphism were found with leptin and glucose levels (p < 0.05), after adjusting for potential covariates. This LEPR polymorphism in the metabolic-syndrome group was also significantly more frequent than in the control group (p < 0.05). However, other gene polymorphisms, LEP G2548A and BDNF Val66Met, showed no significant relationship with leptin levels, BDNF levels or metabolic syndrome. CONCLUSION: These findings suggest leptin levels are linked with metabolic syndrome. LEPR Gln223Arg polymorphism impacted leptin concentrations, and this gene polymorphism may influence susceptibility to metabolic syndrome among Thais. FAU - Suriyaprom, Kanjana AU - Suriyaprom K AD - Faculty of Medical Technology, Rangsit University, Paholyothin Road, Pathumthani 12000, Thailand. kanjana.su@rsu.ac.th. FAU - Tungtrongchitr, Rungsunn AU - Tungtrongchitr R FAU - Thawnasom, Kittisak AU - Thawnasom K LA - eng PT - Journal Article DEP - 20140121 PL - England TA - Diabetol Metab Syndr JT - Diabetology & metabolic syndrome JID - 101488958 PMC - PMC3900466 EDAT- 2014/01/22 06:00 MHDA- 2014/01/22 06:01 PMCR- 2014/01/21 CRDT- 2014/01/22 06:00 PHST- 2013/07/23 00:00 [received] PHST- 2014/01/17 00:00 [accepted] PHST- 2014/01/22 06:00 [entrez] PHST- 2014/01/22 06:00 [pubmed] PHST- 2014/01/22 06:01 [medline] PHST- 2014/01/21 00:00 [pmc-release] AID - 1758-5996-6-6 [pii] AID - 10.1186/1758-5996-6-6 [doi] PST - epublish SO - Diabetol Metab Syndr. 2014 Jan 21;6(1):6. doi: 10.1186/1758-5996-6-6.