PMID- 24444562
OWN - NLM
STAT- MEDLINE
DCOM- 20140904
LR  - 20181203
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 48
IP  - 11
DP  - 2013 Nov
TI  - [Analysis of epidermal growth factor receptor expression and gene expression 
      status in tissue microarray of cervical squamous cell carcinoma].
PG  - 843-6
AB  - OBJECTIVE: To elucidate the protein expression and gene expression status and the 
      relationship between epidermal growth factor receptor (EGFR) protein expression 
      and EGFR gene status. METHODS: Tissue microarray containing 72 cervical squamous 
      cell carcinoma tissues was constructed, and EGFR protein expression and gene 
      status were evaluated by immunohistochemical and fluorescence in situ 
      hybridization (FISH) techniques. RESULTS: Protein expression of EGFR: 69 of 72 
      cervical squamous cell carcinomas were observed. The results demonstrated it was 
      significant association with invasion depth, lymph node metastasis and 
      lymph-vessel invasion (chi(2) = 4.998, P < 0.05; chi(2) = 4.299, P < 0.05; chi(2) = 
      4.686, P < 0.05) in cervical squamous cell carcinomas. For FISH assessing EGFR 
      gene, 64 of 72 carcinomas were observed; 7 of 64 cases showed EGFR gene 
      amplification, and 25 disomy, 23 trisomy and 9 polysomy were detected. There were 
      high levels of protein expression in all the EGFR gene amplification cases, and 
      there were significant association between EGFR protein expression and the gene 
      copy number (chi(2) = 13.564, P < 0.05). CONCLUSIONS: EGFR may participate in the 
      occurrence, progression and metastasis of cervical squamous cell carcinoma. 
      Overexpression of EGFR protein may result from gene amplification and gene copy 
      number increases, which showed that EGFR gene expression status may be a more 
      effective biological indicator of cervical squamous cell carcinoma targeted 
      therapy.
FAU - Zhou, Su-ying
AU  - Zhou SY
AD  - The Third Clinical Collage, Wenzhou Medical University, Wenzhou 325000, China.
FAU - Feng, Guo-fei
AU  - Feng GF
FAU - Chen, Guo-rong
AU  - Chen GR
AD  - 325000 Wenzhou, Department of Pathology, The First Affiliated Hospital of Wenzhou 
      Medical University. Email: chengr1978@aliyun.com.
FAU - Pan, Dan
AU  - Pan D
FAU - Zhang, Pin-nan
AU  - Zhang PN
FAU - Yang, Xiao-min
AU  - Yang XM
FAU - Xia, Zuo-li
AU  - Xia ZL
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/pathology
MH  - ErbB Receptors/genetics/*metabolism
MH  - Female
MH  - Gene Amplification
MH  - *Gene Dosage
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Tissue Array Analysis
MH  - Uterine Cervical Neoplasms/genetics/*metabolism/pathology
EDAT- 2014/01/22 06:00
MHDA- 2014/09/05 06:00
CRDT- 2014/01/22 06:00
PHST- 2014/01/22 06:00 [entrez]
PHST- 2014/01/22 06:00 [pubmed]
PHST- 2014/09/05 06:00 [medline]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2013 Nov;48(11):843-6.