PMID- 24445619
OWN - NLM
STAT- MEDLINE
DCOM- 20140910
LR  - 20140212
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Linking)
VI  - 25
IP  - 4
DP  - 2014 Mar 5
TI  - Antitumor efficacy of argon-helium cryoablation-generated dendritic cell vaccine 
      in glioma.
PG  - 199-204
LID - 10.1097/WNR.0000000000000045 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that play a critical 
      role in priming tumor immune responses. We investigated the mechanisms of 
      antitumor efficacy of DCs pulsed with argon-helium-cryotreated glioma cells. 
      There was significant upregulation of maturation markers (CD80, CD86, MHC-I, and 
      MHC-II) in argon-helium freeze-thawed lysate-pulsed DCs. The concentration of 
      interleukin-6 (IL-6), IL-1beta, tumor necrosis factor-alpha, and IL-12 secreted by 
      lysate-pulsed DCs was increased. The concentration of interferon-gamma secreted by T 
      cells stimulated by lysate-pulsed DCs was increased. The cytotoxicity assay 
      showed that T cells stimulated by lysate-pulsed DCs could kill glioma cells 
      significantly more effectively. Our results suggest that argon-helium 
      freeze-thawed lysate-pulsed DCs in vitro can promote DC maturation and enhance DC 
      antigen-presenting function, and induce cytotoxic T lymphocytes to kill tumor 
      cells. Therefore, the combination of argon-helium cryoablation and DC vaccine may 
      represent a novel treatment method for glioma.
FAU - Yin, Zhilin
AU  - Yin Z
AD  - aDepartment of Neurosurgery, Zhujiang Hospital bThe National Key Clinic 
      Specialty, Key Laboratory on Brain Function Repair and Regeneration, The 
      Neurosurgery Institute of Guangdong Province, Southern Medical University, 
      Guangzhou, China.
FAU - Lu, Guohui
AU  - Lu G
FAU - Xiao, Zhenyong
AU  - Xiao Z
FAU - Liu, Tianzhu
AU  - Liu T
FAU - He, Xiaozheng
AU  - He X
FAU - Wang, Qifu
AU  - Wang Q
FAU - Lin, Chunnan
AU  - Lin C
FAU - Zhang, Shizhong
AU  - Zhang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Cancer Vaccines)
RN  - 0 (Cytokines)
RN  - 206GF3GB41 (Helium)
RN  - 67XQY1V3KH (Argon)
SB  - IM
MH  - Animals
MH  - Argon
MH  - Cancer Vaccines/*therapeutic use
MH  - Cell Death
MH  - Cell Line, Tumor
MH  - Combined Modality Therapy
MH  - Cryosurgery
MH  - Cytokines/metabolism
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Glioma/immunology/*therapy
MH  - Helium
MH  - Lymphocyte Activation
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2014/01/22 06:00
MHDA- 2014/09/11 06:00
CRDT- 2014/01/22 06:00
PHST- 2014/01/22 06:00 [entrez]
PHST- 2014/01/22 06:00 [pubmed]
PHST- 2014/09/11 06:00 [medline]
AID - 10.1097/WNR.0000000000000045 [doi]
PST - ppublish
SO  - Neuroreport. 2014 Mar 5;25(4):199-204. doi: 10.1097/WNR.0000000000000045.