PMID- 24445633
OWN - NLM
STAT- MEDLINE
DCOM- 20140318
LR  - 20210902
IS  - 1526-7598 (Electronic)
IS  - 0003-2999 (Linking)
VI  - 118
IP  - 2
DP  - 2014 Feb
TI  - Up-regulation of NaV1.7 sodium channels expression by tumor necrosis factor-alpha in 
      cultured bovine adrenal chromaffin cells and rat dorsal root ganglion neurons.
PG  - 318-324
LID - 10.1213/ANE.0000000000000085 [doi]
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is not only a key regulator of 
      inflammatory response but also an important pain modulator. TNF-alpha enhances both 
      tetrodotoxin-sensitive (TTX-S) and tetrodotoxin-resistant Na channel currents in 
      dorsal root ganglion (DRG) neurons. However, it remains unknown whether TNF-alpha 
      affects the function and expression of the TTX-S NaV1.7 Na channel, which plays 
      crucial roles in pain generation. METHODS: We used cultured bovine adrenal 
      chromaffin cells expressing the NaV1.7 Na channel isoform and compared them with 
      cultured rat DRG neurons. The expression of TNF receptor 1 and 2 (TNFR1 and 
      TNFR2) in adrenal chromaffin cells was studied by Semiquantitative reverse 
      transcription-polymerase chain reaction. The effects of TNF-alpha on the expression 
      of NaV1.7 were examined with reverse transcription-polymerase chain reaction and 
      Western blot analysis. Results were expressed as mean +/- SEM. RESULTS: TNFR1 and 
      TNFR2 were expressed in adrenal chromaffin cells, as well as reported in DRG 
      neurons. TNF-alpha up-regulated NaV1.7 mRNA by 132% +/- 9% (N = 5, P = 0.004) in 
      adrenal chromaffin cells, as well as 117% +/- 2% (N = 5, P < 0.0001) in DRG 
      neurons. Western blot analysis showed that TNF-alpha increased NaV1.7 protein up to 
      166% +/- 24% (N = 5, corrected P < 0.0001) in adrenal chromaffin cells, 
      concentration- and time-dependently. CONCLUSIONS: TNF-alpha up-regulated NaV1.7 mRNA 
      in both adrenal chromaffin cells and DRG neurons. In addition, TNF-alpha up-regulated 
      the protein expression of the TTX-S NaV1.7 channel in adrenal chromaffin cells. 
      Our findings may contribute to understanding the peripheral nociceptive mechanism 
      of TNF-alpha.
FAU - Tamura, Ryuji
AU  - Tamura R
AD  - From the Departments of Anesthesiology and Intensive Care and Pharmacology, 
      Miyazaki Medical College, University of Miyazaki, Miyazaki, Japan; Department of 
      Anesthesiology, Division of Basic Research, Washington University School of 
      Medicine, St. Louis, Missouri; Department of Sports Health and Welfare, Faculty 
      of Social Welfare, Kyusyu University of Health and Welfare, Miyazaki, Japan.
FAU - Nemoto, Takayuki
AU  - Nemoto T
FAU - Maruta, Toyoaki
AU  - Maruta T
FAU - Onizuka, Shin
AU  - Onizuka S
FAU - Yanagita, Toshihiko
AU  - Yanagita T
FAU - Wada, Akihiko
AU  - Wada A
FAU - Murakami, Manabu
AU  - Murakami M
FAU - Tsuneyoshi, Isao
AU  - Tsuneyoshi I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anesth Analg
JT  - Anesthesia and analgesia
JID - 1310650
RN  - 0 (Actins)
RN  - 0 (NAV1.7 Voltage-Gated Sodium Channel)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type II)
RN  - 0 (Scn9a protein, rat)
RN  - 0 (Tnfrsf1a protein, rat)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - Actins/metabolism
MH  - Adrenal Glands/*metabolism
MH  - Animals
MH  - Cattle
MH  - Chromaffin Cells/*cytology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Ganglia, Spinal/*metabolism
MH  - Male
MH  - NAV1.7 Voltage-Gated Sodium Channel/*metabolism
MH  - Neuralgia/drug therapy
MH  - Neurons/*metabolism
MH  - Rats
MH  - Receptors, Tumor Necrosis Factor, Type I/metabolism
MH  - Receptors, Tumor Necrosis Factor, Type II/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium/chemistry
MH  - Tetrodotoxin/chemistry
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Up-Regulation
EDAT- 2014/01/22 06:00
MHDA- 2014/03/19 06:00
CRDT- 2014/01/22 06:00
PHST- 2014/01/22 06:00 [entrez]
PHST- 2014/01/22 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - 00000539-201402000-00013 [pii]
AID - 10.1213/ANE.0000000000000085 [doi]
PST - ppublish
SO  - Anesth Analg. 2014 Feb;118(2):318-324. doi: 10.1213/ANE.0000000000000085.