PMID- 24446377
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20140121
IS  - 1099-1352 (Electronic)
IS  - 0952-3499 (Linking)
VI  - 27
IP  - 3
DP  - 2014 Mar
TI  - Spectroscopic studies on the interaction mechanisms of safranin T with herring 
      sperm DNA using acridine orange as a fluorescence probe.
PG  - 131-7
LID - 10.1002/jmr.2341 [doi]
AB  - Under the condition of physiological pH environment (pH = 7.40), the interactions 
      of safranin T (ST) with herring sperm DNA were studied by means of spectral 
      methods using acridine orange (AO) as a fluorescence probe. The spectroscopic 
      characteristics of DNA-AO in the case of ST (along with the increase of 
      concentration) were observed in an aqueous medium. The binding constants for ST 
      stranded DNA and competitive bindings of ST interacting with DNA-AO systems were 
      examined by fluorescence spectra, and the binding mechanism of ST with DNA was 
      researched via viscosity measurements. All the testimony manifested that bonding 
      modes between ST and DNA were evidenced to be intercalative binding and 
      electrostatic binding, and the combining constant of ST with DNA was obtained. 
      The binding of ST to DNA was driven by entropy and enthalpy through the 
      calculated thermodynamic parameters (Deltar Hm (Ө), Deltar Sm and Deltar Gm (Ө)).
CI  - Copyright (c) 2014 John Wiley & Sons, Ltd.
FAU - Long, Jun
AU  - Long J
AD  - School of Materials Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010, China.
FAU - Wang, Xing-ming
AU  - Wang XM
FAU - Xu, Dong-ling
AU  - Xu DL
FAU - Ding, Li-sheng
AU  - Ding LS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Recognit
JT  - Journal of molecular recognition : JMR
JID - 9004580
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Intercalating Agents)
RN  - 0 (Phenazines)
RN  - 0 (Solutions)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
RN  - XTX0YXU2HV (safranine T)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Animals
MH  - Antineoplastic Agents/*chemistry
MH  - Binding, Competitive
MH  - DNA/*chemistry/isolation & purification
MH  - Fishes
MH  - Fluorescent Dyes/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Intercalating Agents/*chemistry
MH  - Kinetics
MH  - Male
MH  - Phenazines/*chemistry
MH  - Solutions
MH  - Spectrometry, Fluorescence
MH  - Spectrophotometry, Ultraviolet
MH  - Spermatozoa/chemistry
MH  - Static Electricity
MH  - Thermodynamics
OTO - NOTNLM
OT  - DNA
OT  - acridine orange
OT  - fluorescence spectra
OT  - interaction
OT  - safranin T
EDAT- 2014/01/22 06:00
MHDA- 2014/09/03 06:00
CRDT- 2014/01/22 06:00
PHST- 2013/09/16 00:00 [received]
PHST- 2013/11/01 00:00 [revised]
PHST- 2013/11/13 00:00 [accepted]
PHST- 2014/01/22 06:00 [entrez]
PHST- 2014/01/22 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
AID - 10.1002/jmr.2341 [doi]
PST - ppublish
SO  - J Mol Recognit. 2014 Mar;27(3):131-7. doi: 10.1002/jmr.2341.