PMID- 24449957
OWN - NLM
STAT- MEDLINE
DCOM- 20140812
LR  - 20190923
IS  - 1442-2042 (Electronic)
IS  - 0919-8172 (Linking)
VI  - 3
IP  - 1 Suppl
DP  - 1996 Jan
TI  - Microchimerism in female renal transplant recipients from male donors.
PG  - S114-9
AB  - Microchimerism in 23 female renal transplant recipients from male donors was 
      studied using nested polymerase chain reaction (nPCR) and fluorescence in situ 
      hybridization (FISH) to detect Y-chromosome. nPCR was a sensitive and specific 
      assay enabling a detection rate of 1/10(6) male/female cells, compared with a 
      sensitivity of 1/10(2) by standard PCR (sPCR). None of the 23 patients with a 
      male allograft demonstrated Y-chromosome using sPCR. In contrast, 1 3 (56.5%) 
      patients demonstrated Y-chromosome with nPCR. Of 9 patients proven to have 
      microchimerism by nPCR, only 3 also demonstrated Y-chromosome using FISH. The 
      existence of B cells and CD8 cells in donor chimeric cells were proved by 
      separation with Dynabeads class I and class II. Dynamic changes of microchimerism 
      occurred in 4 of 5 patients. Four patients were proven to have microchimerism 
      within a year of transplantation and the microchimerism later disappeared in 3, 
      although the sequential change was variable in individual patients. There was no 
      correlation between microchimerism and patients'clinical factors such as 
      donor-specific blood transfusion, HLA matching, immunosuppression, past history 
      of acute rejection and chronic rejection. The degree of microchimerism in renal 
      transplant recipients was relatively low, and its existence did not seem to be 
      compatible with long-term graft acceptance. However, further studies are required 
      to elucidate the immunological mechanism of microchimerism, and it might be an 
      important clue to immunological tolerance.
FAU - Aikawa, A
AU  - Aikawa A
FAU - Yamashita, M
AU  - Yamashita M
FAU - Arai, K
AU  - Arai K
FAU - Hirayama, N
AU  - Hirayama N
FAU - Ohara, T
AU  - Ohara T
FAU - Hasegawa, A
AU  - Hasegawa A
FAU - Kaneshige, T
AU  - Kaneshige T
FAU - Hi rai, H
AU  - Hi rai H
FAU - Goto, T
AU  - Goto T
FAU - Fuj imura, Y
AU  - Fuj imura Y
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Int J Urol
JT  - International journal of urology : official journal of the Japanese Urological 
      Association
JID - 9440237
SB  - IM
MH  - Acute Disease
MH  - B-Lymphocytes/immunology
MH  - *Chimerism
MH  - *Chromosomes, Human, Y
MH  - Chronic Disease
MH  - Female
MH  - Graft Rejection/*genetics/*immunology
MH  - Humans
MH  - Immune Tolerance/genetics/immunology
MH  - In Situ Hybridization, Fluorescence
MH  - *Kidney Transplantation
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Sex Factors
MH  - Tissue Donors
MH  - Transplantation Chimera/*immunology
EDAT- 1996/01/01 00:00
MHDA- 2014/08/13 06:00
CRDT- 2014/01/24 06:00
PHST- 2014/01/24 06:00 [entrez]
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2014/08/13 06:00 [medline]
AID - 10.1111/j.1442-2042.1996.tb00328.x [doi]
PST - ppublish
SO  - Int J Urol. 1996 Jan;3(1 Suppl):S114-9. doi: 10.1111/j.1442-2042.1996.tb00328.x.