PMID- 24450108
OWN - NLM
STAT- MEDLINE
DCOM- 20140313
LR  - 20140123
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 61
IP  - 11
DP  - 2013 Nov
TI  - [HLA genotyping for molecular epidemiological analysis of humans].
PG  - 1026-34
AB  - The major histocompatibility complex (MHC) gene family in humans is termed the 
      human leukocyte antigen (HLA) locus, and is one of the most polymorphic loci in 
      the human genome. HLA and related genes play many important roles in the immune 
      system. HLA matching is critical for the success of organ and hematopoietic stem 
      cell transplantation. Additionally, HLA genes and proteins are associated with 
      autoimmunity, immunity to infections, tumor development, and adverse drug 
      reactions (ADR). For matching donor and recipient HLA types and to understand the 
      role played by the HLA in disease development, lymphocyte cytotoxicity tests 
      (LCT) were developed by Dr. Terasaki in the 1960s. This serological typing 
      method, which was in use until recently, is now no longer used and has been 
      replaced by DNA-based typing methods such as PCR-SSP (sequence-specific primers), 
      PCR-rSSO(reverse sequence-specific oligonucleotide probes), and PCR-SBT 
      (sequence-based typing). In the future, the current HLA-typing methods will be 
      replaced by faster, more detailed and more cost-effective methods such as 
      next-generation sequencing (NGS). In this review, we focus on the past and 
      present methods of HLA typing, and the role of the HLA in various diseases.
FAU - Azuma, Fumihiro
AU  - Azuma F
AD  - HLA Laboratory, Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Koto-ku, 
      Tokyo 135-8639, Japan.
FAU - Kashiwase, Koichi
AU  - Kashiwase K
AD  - HLA Laboratory, Japanese Red Cross Kanto-Koshinetsu Block Blood Center, Koto-ku, 
      Tokyo 135-8639, Japan.
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
RN  - 0 (HLA Antigens)
SB  - IM
MH  - *Genetic Predisposition to Disease
MH  - Genome, Human/genetics
MH  - Genotype
MH  - HLA Antigens/analysis/*genetics
MH  - Humans
MH  - Polymorphism, Genetic/genetics
MH  - Sequence Analysis, DNA/methods
EDAT- 2014/01/24 06:00
MHDA- 2014/03/14 06:00
CRDT- 2014/01/24 06:00
PHST- 2014/01/24 06:00 [entrez]
PHST- 2014/01/24 06:00 [pubmed]
PHST- 2014/03/14 06:00 [medline]
PST - ppublish
SO  - Rinsho Byori. 2013 Nov;61(11):1026-34.