PMID- 24450815
OWN - NLM
STAT- MEDLINE
DCOM- 20150722
LR  - 20141203
IS  - 1469-0691 (Electronic)
IS  - 1198-743X (Linking)
VI  - 20
IP  - 10
DP  - 2014 Oct
TI  - A novel fluorescence in situ hybridization test for rapid pathogen identification 
      in positive blood cultures.
PG  - O760-3
LID - 10.1111/1469-0691.12561 [doi]
AB  - A novel molecular beacon-based fluorescence in situ hybridization (FISH) test 
      allowing for the identification of a wide range of bacterial pathogens directly 
      in positive blood cultures (BCs) was evaluated with positive BCs of 152 patients. 
      Depending on the Gram stain, either a Gram-negative or a Gram-positive panel was 
      used. The time to result was 30 min, and the hands-on time was only 10 min. Seven 
      per cent of the cultured microorganisms were not included in the FISH panels; the 
      identification rate of those included was 95.2%. Overall, the FISH test enabled 
      accurate pathogen identification in 88.2% of all cases analysed.
CI  - (c) 2014 The Authors Clinical Microbiology and Infection (c) 2014 European Society of 
      Clinical Microbiology and Infectious Diseases.
FAU - Makristathis, A
AU  - Makristathis A
AD  - Division of Clinical Microbiology, Department of Laboratory Medicine, Medical 
      University Vienna, Vienna, Austria.
FAU - Riss, S
AU  - Riss S
FAU - Hirschl, A M
AU  - Hirschl AM
LA  - eng
PT  - Journal Article
DEP - 20140220
PL  - England
TA  - Clin Microbiol Infect
JT  - Clinical microbiology and infection : the official publication of the European 
      Society of Clinical Microbiology and Infectious Diseases
JID - 9516420
SB  - IM
MH  - Bacteria/classification/*isolation & purification
MH  - Blood/*microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Typing
MH  - Sensitivity and Specificity
MH  - Sepsis/blood/*diagnosis/microbiology
OTO - NOTNLM
OT  - Blood culture
OT  - fluorescence in situ hybridization
OT  - identification
OT  - pathogen
EDAT- 2014/01/24 06:00
MHDA- 2015/07/23 06:00
CRDT- 2014/01/24 06:00
PHST- 2013/08/27 00:00 [received]
PHST- 2013/12/05 00:00 [revised]
PHST- 2014/01/17 00:00 [accepted]
PHST- 2014/01/24 06:00 [entrez]
PHST- 2014/01/24 06:00 [pubmed]
PHST- 2015/07/23 06:00 [medline]
AID - S1198-743X(14)65417-3 [pii]
AID - 10.1111/1469-0691.12561 [doi]
PST - ppublish
SO  - Clin Microbiol Infect. 2014 Oct;20(10):O760-3. doi: 10.1111/1469-0691.12561. Epub 
      2014 Feb 20.