PMID- 24451028
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20141121
LR  - 20220321
IS  - 1386-6346 (Print)
IS  - 1386-6346 (Linking)
VI  - 44
IP  - 13
DP  - 2014 Dec
TI  - Plasma minichromosome maintenance complex component 6 is a novel biomarker for 
      hepatocellular carcinoma patients.
PG  - 1347-56
LID - 10.1111/hepr.12303 [doi]
AB  - AIM: This study aimed to investigate the presence of plasma minichromosome 
      maintenance complex component 6 (MCM6) mRNA and protein levels in hepatocellular 
      carcinoma (HCC) patients and evaluate their diagnostic value for HCC. METHODS: 
      Blood samples were collected from 61 HCC and 29 cirrhotic patients, and 30 
      healthy individuals. Circulating RNA was extracted from plasma of all samples. 
      The mRNA for MCM6 were amplified and quantified by real-time polymerase chain 
      reaction. Plasma MCM6 and alpha-fetoprotein (AFP) protein levels were measured by 
      enzyme-linked immunosorbent assay. RESULTS: In HCC patients, MCM6 mRNA and 
      protein levels were significantly increased over the cirrhotic and healthy 
      controls. The levels of MCM6 mRNA and protein in the plasma of HCC patients 
      correlated to vascular invasion (P < 0.01). Higher MCM6 protein levels also 
      correlated with tumor stage progression and lymph node metastasis. The MCM6 
      protein has sensitivity of 67.2% and specificity of 89.8% in differentiating 
      total HCC from non-HCC individuals. In the AFP negative HCC group, MCM6 mRNA and 
      protein could both detect 76.9% of HCC patients; combining the two of them 
      increased the detection rate to 84.6%. In small HCC patients, MCM6 mRNA and 
      protein could detect 64.3% and 71.4% of patients, respectively; combining AFP, 
      MCM6 mRNA and MCM6 protein could detect 85.7% of small HCC patients. CONCLUSION: 
      Our results suggest that MCM6 mRNA and protein levels in plasma can be promising 
      independent biomarkers for HCC, especially in AFP negative and small HCC 
      patients.
CI  - (c) 2014 The Japan Society of Hepatology.
FAU - Zheng, Tenghao
AU  - Zheng T
AD  - Department of Gastroenterology and Hepatology, People's Hospital of Zhengzhou 
      University, Zhengzhou, China.
FAU - Chen, Ming
AU  - Chen M
FAU - Han, Shuangyin
AU  - Han S
FAU - Zhang, Lida
AU  - Zhang L
FAU - Bai, Yangqiu
AU  - Bai Y
FAU - Fang, Xinhui
AU  - Fang X
FAU - Ding, Song-Ze
AU  - Ding SZ
FAU - Yang, Yuxiu
AU  - Yang Y
LA  - eng
PT  - Journal Article
DEP - 20140228
PL  - Netherlands
TA  - Hepatol Res
JT  - Hepatology research : the official journal of the Japan Society of Hepatology
JID - 9711801
OTO - NOTNLM
OT  - circulating nucleic acids
OT  - enzyme-linked immunosorbent assay
OT  - hepatocellular carcinoma
OT  - minichromosome maintenance complex component 6
OT  - real-time quantitative polymerase chain reaction
EDAT- 2014/01/24 06:00
MHDA- 2014/01/24 06:01
CRDT- 2014/01/24 06:00
PHST- 2013/09/06 00:00 [received]
PHST- 2014/01/08 00:00 [revised]
PHST- 2014/01/16 00:00 [accepted]
PHST- 2014/01/24 06:00 [entrez]
PHST- 2014/01/24 06:00 [pubmed]
PHST- 2014/01/24 06:01 [medline]
AID - 10.1111/hepr.12303 [doi]
PST - ppublish
SO  - Hepatol Res. 2014 Dec;44(13):1347-56. doi: 10.1111/hepr.12303. Epub 2014 Feb 28.