PMID- 24451989
OWN - NLM
STAT- MEDLINE
DCOM- 20141104
LR  - 20140314
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 90
IP  - 3
DP  - 2014 Mar
TI  - Expression and regulation of MMP1, MMP3, and MMP9 in the chicken ovary in 
      response to gonadotropins, sex hormones, and TGFB1.
PG  - 57
LID - 10.1095/biolreprod.113.114249 [doi]
AB  - Matrix metalloproteinases (MMPs) are a specific class of proteolytic enzymes that 
      play critical roles in follicular development and luteinization in mammals. 
      However, the role of MMPs in avian ovary remains largely unknown. We found that 
      three MMP genes (MMP1, MMP3, and MMP9) were significantly up-regulated in 
      23-wk-old (laying phase) chicken ovaries compared with 6-wk-old ovaries 
      (prepubertal phase). In reproductively active chicken ovary, MMP1 expression 
      (both mRNA and protein) remained low in prehierarchical and preovulatory 
      follicles but increased in postovulatory follicles (POFs). Both MMP3 and MMP9 
      expression levels increased during follicular maturation. MMP3 reached maximal 
      expression in the first largest follicle (F1), while MMP9 levels continued to 
      rise in POF1 and POF2 after ovulation. Immunohistochemistry, Western blot 
      analysis, and zymography experiments indicated that MMP1, MMP3, and MMP9 were 
      synthesized and secreted by granulosa cells of different follicles in the chicken 
      ovary. The mRNA expression of MMP1 and MMP3 in the granulosa cells was stimulated 
      by follicle-stimulating hormone, luteinizing hormone, progesterone, and estrogen 
      but not by transforming growth factor beta 1 (TGFB1). However, the mRNA of MMP9 
      was induced by TGFB1 but not follicle-stimulating hormone, luteinizing hormone, 
      progesterone, or estrogen. Luciferase reporter and mutagenesis analysis indicated 
      the AP1 and NFkappaB elements located in the promoter region from -1700 to -2400 
      bp were critical for both basal and TGFB1-induced MMP9 transcription. These data 
      provide the first spatial-temporal expression analysis of MMP system in the 
      chicken ovary.
FAU - Zhu, Guiyu
AU  - Zhu G
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Animal Science and Veterinary Medicine, Shandong 
      Agricultural University, Taian, China.
FAU - Kang, Li
AU  - Kang L
FAU - Wei, Qingqing
AU  - Wei Q
FAU - Cui, Xinxing
AU  - Cui X
FAU - Wang, Shouzhi
AU  - Wang S
FAU - Chen, Yuxia
AU  - Chen Y
FAU - Jiang, Yunliang
AU  - Jiang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140313
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Gonadotropins)
RN  - 0 (NF-kappa B)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 63231-63-0 (RNA)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chickens/*physiology
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Gonadal Steroid Hormones/*pharmacology
MH  - Gonadotropins/*pharmacology
MH  - Granulosa Cells/drug effects/metabolism
MH  - Immunohistochemistry
MH  - Matrix Metalloproteinase 1/*biosynthesis/genetics
MH  - Matrix Metalloproteinase 3/*biosynthesis/genetics
MH  - Matrix Metalloproteinase 9/*biosynthesis/genetics
MH  - Mutagenesis
MH  - NF-kappa B/biosynthesis/genetics
MH  - Ovarian Follicle/drug effects
MH  - Ovary/*drug effects/*enzymology
MH  - Plasmids/genetics
MH  - RNA/biosynthesis/isolation & purification
MH  - Real-Time Polymerase Chain Reaction
MH  - Transcription Factor AP-1/biosynthesis/genetics
MH  - Transfection
MH  - Transforming Growth Factor beta1/*pharmacology
OTO - NOTNLM
OT  - MMP
OT  - chicken
OT  - domestic animal reproduction
OT  - extracellular matrix
OT  - follicle
OT  - granulosa cell
OT  - ovary
EDAT- 2014/01/24 06:00
MHDA- 2014/11/05 06:00
CRDT- 2014/01/24 06:00
PHST- 2014/01/24 06:00 [entrez]
PHST- 2014/01/24 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
AID - biolreprod.113.114249 [pii]
AID - 10.1095/biolreprod.113.114249 [doi]
PST - epublish
SO  - Biol Reprod. 2014 Mar 13;90(3):57. doi: 10.1095/biolreprod.113.114249. Print 2014 
      Mar.