PMID- 24452841
OWN - NLM
STAT- MEDLINE
DCOM- 20140826
LR  - 20211021
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Linking)
VI  - 59
IP  - 7
DP  - 2014 Jul
TI  - Bone marrow cells enhance liver regeneration after massive hepatectomy in mice.
PG  - 1484-9
LID - 10.1007/s10620-014-3032-7 [doi]
AB  - BACKGROUND: Recent evidence indicates that transplanted autologous bone marrow 
      cells (BMCs) can be converted into functional liver cells. BMC therapy can 
      improve hepatic function and increase the potential for liver regeneration in 
      patients with serious liver damage. We investigated whether BMC therapy 
      influenced liver regeneration after massive hepatectomy in mice. METHODS: Male 
      C57/BL6 mice underwent 70 % hepatectomy, followed by injection of BMCs via the 
      portal vein (PV group), BMCs via the tail vein (IV group), or saline via the 
      portal vein (control group). Analysis of serum enzyme levels and liver histology 
      was performed on postoperative days (POD) 1, 3, and 5. RESULTS: Compared with the 
      control group, the rate of liver regeneration on POD 3 and 5 was significantly 
      higher in the PV group, but not in the IV group. Examination of the mitotic index 
      and Ki-67 labeling index revealed that the increased liver regeneration resulted 
      from stimulation of DNA synthesis. On POD 3, the serum levels of interleukin 
      (IL)-6 and hepatocyte growth factor (HGF) were significantly higher and the 
      expression of IL-6 and HGF mRNA in the remnant liver tended to be higher in the 
      PV group than in the control group. Histological examination showed BMCs in the 
      liver of the PV group, as well as conversion of BMCs into liver cells. 
      CONCLUSIONS: Our findings indicate that the injection of BMCs via the portal 
      vein, but not the injection of BMCs via the tail, enhances liver regeneration 
      after massive hepatectomy in mice.
FAU - Kaibori, Masaki
AU  - Kaibori M
AD  - Department of Surgery, Hirakata Hospital, Kansai Medical University, 2-3-1 
      Shinmachi, Hirakata, Osaka, 573-1191, Japan, kaibori@hirakata.kmu.ac.jp.
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Shimo, Tomohiko
AU  - Shimo T
FAU - Ishizaki, Morihiko
AU  - Ishizaki M
FAU - Matsui, Kosuke
AU  - Matsui K
FAU - Tanaka, Yoshito
AU  - Tanaka Y
FAU - Ohishi, Masaharu
AU  - Ohishi M
FAU - Araki, Yoshiro
AU  - Araki Y
FAU - Tokuhara, Katsuji
AU  - Tokuhara K
FAU - Okumura, Tadayoshi
AU  - Okumura T
FAU - Nishizawa, Mikio
AU  - Nishizawa M
FAU - Kwon, A-Hon
AU  - Kwon AH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20140123
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Biomarkers)
RN  - 0 (HGF protein, mouse)
RN  - 0 (Interleukin-6)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
CIN - Dig Dis Sci. 2015 Jan;60(1):280-2. PMID: 25480406
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Transplantation/*methods
MH  - *Hepatectomy
MH  - Hepatocyte Growth Factor/metabolism
MH  - Interleukin-6/metabolism
MH  - Liver/metabolism/pathology/surgery
MH  - *Liver Regeneration
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Postoperative Care/*methods
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transplantation, Autologous
MH  - Treatment Outcome
EDAT- 2014/01/24 06:00
MHDA- 2014/08/27 06:00
CRDT- 2014/01/24 06:00
PHST- 2013/02/17 00:00 [received]
PHST- 2014/01/08 00:00 [accepted]
PHST- 2014/01/24 06:00 [entrez]
PHST- 2014/01/24 06:00 [pubmed]
PHST- 2014/08/27 06:00 [medline]
AID - 10.1007/s10620-014-3032-7 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2014 Jul;59(7):1484-9. doi: 10.1007/s10620-014-3032-7. Epub 2014 Jan 
      23.