PMID- 24456068
OWN - NLM
STAT- MEDLINE
DCOM- 20150304
LR  - 20211021
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 20
IP  - 13-14
DP  - 2014 Jul
TI  - Dynamic vibration cooperates with connective tissue growth factor to modulate 
      stem cell behaviors.
PG  - 1922-34
LID - 10.1089/ten.TEA.2013.0496 [doi]
AB  - Vocal fold disorders affect 3-9% of the U.S. population. Tissue engineering 
      offers an alternative strategy for vocal fold repair. Successful engineering of 
      vocal fold tissues requires a strategic combination of therapeutic cells, 
      biomimetic scaffolds, and physiologically relevant mechanical and biochemical 
      factors. Specifically, we aim to create a vocal fold-like microenvironment to 
      coax stem cells to adopt the phenotype of vocal fold fibroblasts (VFFs). Herein, 
      high frequency vibratory stimulations and soluble connective tissue growth factor 
      (CTGF) were sequentially introduced to mesenchymal stem cells (MSCs) cultured on 
      a poly(varepsilon-caprolactone) (PCL)-derived microfibrous scaffold for a total of 6 days. 
      The initial 3-day vibratory culture resulted in an increased production of 
      hyaluronic acids (HA), tenascin-C (TNC), decorin (DCN), and matrix 
      metalloproteinase-1 (MMP1). The subsequent 3-day CTGF treatment further enhanced 
      the cellular production of TNC and DCN, whereas CTGF treatment alone without the 
      vibratory preconditioning significantly promoted the synthesis of collagen I (Col 
      1) and sulfated glycosaminoglycans (sGAGs). The highest level of MMP1, TNC, Col 
      III, and DCN production was found for cells being exposed to the combined 
      vibration and CTGF treatment. Noteworthy, the vibration and CTGF elicited a 
      differential stimulatory effect on elastin (ELN), HA synthase 1 (HAS1), and 
      fibroblast-specific protein-1 (FSP-1). The mitogenic activity of CTGF was only 
      elicited in naive cells without the vibratory preconditioning. The combined 
      treatment had profound, but opposite effects on mitogen-activated protein kinase 
      (MAPK) pathways, Erk1/2 and p38, and the Erk1/2 pathway was critical for the 
      observed mechano-biochemical responses. Collectively, vibratory stresses and CTGF 
      signals cooperatively coaxed MSCs toward a VFF-like phenotype and accelerated the 
      synthesis and remodeling of vocal fold matrices.
FAU - Tong, Zhixiang
AU  - Tong Z
AD  - 1 Department of Materials Science and Engineering, Delaware Biotechnology 
      Institute, University of Delaware , Newark, Delaware.
FAU - Zerdoum, Aidan B
AU  - Zerdoum AB
FAU - Duncan, Randall L
AU  - Duncan RL
FAU - Jia, Xinqiao
AU  - Jia X
LA  - eng
GR  - P20 GM103446/GM/NIGMS NIH HHS/United States
GR  - R01 DC011377/DC/NIDCD NIH HHS/United States
GR  - R01008965/PHS HHS/United States
GR  - R01DC011377/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140227
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Butadienes)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Nitriles)
RN  - 0 (U 0126)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Biomechanical Phenomena/drug effects
MH  - Bioreactors
MH  - Blotting, Western
MH  - Butadienes/pharmacology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Connective Tissue Growth Factor/*pharmacology
MH  - Extracellular Matrix Proteins/drug effects/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/drug effects/enzymology
MH  - Nitriles/pharmacology
MH  - Time Factors
MH  - *Vibration
PMC - PMC4086222
EDAT- 2014/01/25 06:00
MHDA- 2015/03/05 06:00
PMCR- 2015/07/01
CRDT- 2014/01/25 06:00
PHST- 2014/01/25 06:00 [entrez]
PHST- 2014/01/25 06:00 [pubmed]
PHST- 2015/03/05 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2013.0496 [pii]
AID - 10.1089/ten.TEA.2013.0496 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2014 Jul;20(13-14):1922-34. doi: 10.1089/ten.TEA.2013.0496. 
      Epub 2014 Feb 27.