PMID- 24456192
OWN - NLM
STAT- MEDLINE
DCOM- 20140828
LR  - 20181202
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 116
IP  - 4
DP  - 2014 Apr
TI  - Investigation of the mechanisms underlying the high acetylene-reducing activity 
      exhibited by the soil bacterial community from BC2 horizon in the permafrost zone 
      of the East Siberian larch forest bed.
PG  - 865-76
LID - 10.1111/jam.12424 [doi]
AB  - AIMS: This study addressed a microbial composition of East Siberian larch forest 
      bed soil and focused on major nitrogen-fixing bacterial consortia showing the 
      highest capacity to induce acetylene reduction. METHODS AND RESULTS: The ability 
      of a soil microbial consortium to effectively reduce acetylene was investigated 
      using gellan gum medium. In parallel, each microbial component in the medium was 
      analysed by a 16S rRNA gene-targeting denaturing gradient gel electrophoresis 
      (DGGE), together with an attempt to isolate pure culture of the culturable 
      eubacteria. Six strains of the isolated bacteria were characterized as 
      diazotrophs, and Burkholderia xenovorans was found to be one of the major 
      nitrogen fixer in the bacterial community cultured from the BC2 horizon soil 
      microbiota. Co-culturing of either a diazotrophic Pseudomonas sp. with a 
      nondiazotrophic Luteibacter sp. or a diazotrophic Luteibacter sp. with a 
      nondiazotrophic bacterium B. xenovorans resulted in a marked elevation in 
      acetylene reduction over that observed in the pure culture of each diazotroph. 
      CONCLUSIONS: A promotion of acetylene reduction in mixed bacterial cultures, 
      particularly when the cultures included Luteibacter, was clearly observed due to 
      an increase in the bacterial cell population. SIGNIFICANCE AND IMPACT OF THE 
      STUDY: Our study, which showed that some unculturable bacteria including 
      anaerobic Clostridium sp. that can provide major contribution to nitrogen 
      fixation, may provide key information useful in identifying the 'missing link' in 
      the nitrogen cycle in the cryogenic soil ecosystem of boreal forests.
CI  - (c) 2013 The Society for Applied Microbiology.
FAU - Hara, S
AU  - Hara S
AD  - Graduate School and Research Faculty of Agriculture, Hokkaido University, 
      Sapporo, Hokkaido, Japan.
FAU - Desyatkin, R V
AU  - Desyatkin RV
FAU - Hashidoko, Y
AU  - Hashidoko Y
LA  - eng
SI  - GENBANK/AB379689
SI  - GENBANK/AB379690
SI  - GENBANK/AB379692
SI  - GENBANK/AB627008
SI  - GENBANK/AB627009
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140124
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (RNA, Ribosomal, 16S)
RN  - N762921K75 (Nitrogen)
RN  - OC7TV75O83 (Acetylene)
SB  - IM
MH  - Acetylene/*metabolism
MH  - Bacteria/genetics/isolation & purification/*metabolism
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Ecosystem
MH  - Freezing
MH  - Larix
MH  - Molecular Sequence Data
MH  - Nitrogen/metabolism
MH  - *Nitrogen Fixation
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Soil Microbiology
MH  - Trees
OTO - NOTNLM
OT  - Luteibacter
OT  - Siberian Taiga forest bed soil microbes
OT  - missing link of nitrogen circulation
OT  - nitrogen fixation
OT  - synergistic cell growth promotion
EDAT- 2014/01/25 06:00
MHDA- 2014/08/29 06:00
CRDT- 2014/01/25 06:00
PHST- 2013/09/02 00:00 [received]
PHST- 2013/12/02 00:00 [revised]
PHST- 2013/12/09 00:00 [accepted]
PHST- 2014/01/25 06:00 [entrez]
PHST- 2014/01/25 06:00 [pubmed]
PHST- 2014/08/29 06:00 [medline]
AID - 10.1111/jam.12424 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2014 Apr;116(4):865-76. doi: 10.1111/jam.12424. Epub 2014 Jan 
      24.