PMID- 24457640
OWN - NLM
STAT- MEDLINE
DCOM- 20141121
LR  - 20231206
IS  - 1689-0035 (Electronic)
IS  - 0065-1400 (Linking)
VI  - 73
IP  - 4
DP  - 2013
TI  - Leukemia inhibitory factor inhibits the proliferation of primary rat astrocytes 
      induced by oxygen-glucose deprivation.
PG  - 485-94
LID - 1954 [pii]
LID - 10.55782/ane-2013-1954 [doi]
AB  - Leukemia inhibitory factor (LIF) is a neuroprotective cytokine that is necessary 
      for the normal development of astrocytes. Oxygen-glucose deprivation (OGD) can 
      induce astrocyte proliferation by increasing hypoxia-inducible factor alpha 
      (HIF-1alpha) and vascular endothelial growth factor (VEGF). Here, we studied 
      whether LIF affects the proliferation of cultured primary rat astrocytes under 
      OGD conditions by measuring EdU incorporation into astrocyte DNA and the 
      expression of proliferating cell nuclear antigen (PCNA) mRNA and protein. Our 
      findings show that low concentrations of LIF (5 and 10 ng/mL) significantly 
      decreased EdU incorporation and downregulated the expression of PCNA mRNA and 
      PCNA protein in astrocytes subjected to OGD. A low concentration of LIF (10 
      ng/mL) clearly inhibited astrocyte proliferation induced by OGD, while a higher 
      concentration (50 ng/mL) had no effect. To investigate the mechanism of this 
      inhibition by LIF (10 ng/mL), the expression of 3 related genes (LIF receptor, 
      HIF-1alpha, and VEGF) was assessed using real-time PCR; VEGF protein expression 
      was measured by Western blot. Our results indicate that LIFR mRNA was 
      downregulated in astrocytes subjected to OGD. Interestingly, treatment with LIF 
      further reduced LIFR mRNA expression in these cells. LIF treatment also decreased 
      the expression of HIF-1alpha mRNA, VEGF mRNA, and VEGF protein induced by OGD. 
      Low concentrations of LIF were observed to inhibit astrocyte proliferation 
      induced by OGD.
FAU - Fan, Yu-Ying
AU  - Fan YY
AD  - Department of Pediatrics, Shengjing Hospital of China Medical University, 
      Shenyang, China, wangh1@sj-hospital.org.
FAU - Zhang, Jun-Mei
AU  - Zhang JM
FAU - Wang, Hua
AU  - Wang H
FAU - Liu, Xue-Yan
AU  - Liu XY
FAU - Yang, Feng-Hua
AU  - Yang FH
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Acta Neurobiol Exp (Wars)
JT  - Acta neurobiologiae experimentalis
JID - 1246675
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Astrocytes/cytology/*drug effects
MH  - Cell Hypoxia/drug effects
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects/physiology
MH  - Cells, Cultured
MH  - Glucose/*metabolism
MH  - Leukemia Inhibitory Factor/*pharmacology
MH  - Oxygen/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Vascular Endothelial Growth Factor A/*metabolism
EDAT- 2014/01/25 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/01/25 06:00
PHST- 2014/01/25 06:00 [entrez]
PHST- 2014/01/25 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 1954 [pii]
AID - 10.55782/ane-2013-1954 [doi]
PST - ppublish
SO  - Acta Neurobiol Exp (Wars). 2013;73(4):485-94. doi: 10.55782/ane-2013-1954.