PMID- 2445772
OWN - NLM
STAT- MEDLINE
DCOM- 19880115
LR  - 20240104
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 17
IP  - 3-4
DP  - 1987 Sep
TI  - Comparison of enzyme immunoassay and reverse transcriptase assay for detection of 
      HIV in culture supernates.
PG  - 237-45
AB  - An enzyme immunoassay (EIA) was developed for detection of human immunodeficiency 
      virus antigen (HIV Ag) in tissue culture supernatants. The assay was found to be 
      specific for HIV and cheaper, easier to perform and more sensitive than the 
      generally used reverse transcriptase (RT) assay. Cultures of peripheral blood 
      leucocytes (PBL) from 106 patients with acquired immune deficiency syndrome 
      (AIDS), AIDS related complex (ARC), healthy anti-HIV positive subjects and 
      healthy anti-HIV negative subjects were held for 35 days and the supernatant 
      fluid tested at regular intervals by EIA and RT. Of these 106 cultures, the 
      presence of HIV was detected by EIA in 27 and by RT in 21. While six cultures 
      were positive by EIA alone, none were positive by RT alone; the specificity of 
      the results in the six EIA positive RT negative cultures was confirmed by 
      subculture. In the 21 cultures in which HIV was detected by both techniques, the 
      EIA became positive first on 10 occasions; in the remaining cultures both tests 
      became positive at the same time. The HIV Ag assay reduces the time taken to 
      process specimens and thus increases the efficiency and reduces the cost of 
      isolation procedures.
FAU - Healey, D S
AU  - Healey DS
AD  - National HIV Reference Laboratory, Fairfield Hospital for Communicable Diseases, 
      Victoria, Australia.
FAU - Jowett, J B
AU  - Jowett JB
FAU - Beaton, F
AU  - Beaton F
FAU - Maskill, W J
AU  - Maskill WJ
FAU - Gust, I D
AU  - Gust ID
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antigens, Viral)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Antigens, Viral/*analysis
MH  - Cells, Cultured
MH  - HIV/enzymology/immunology/*isolation & purification
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - RNA-Directed DNA Polymerase/*analysis
EDAT- 1987/09/01 00:00
MHDA- 1987/09/01 00:01
CRDT- 1987/09/01 00:00
PHST- 1987/09/01 00:00 [pubmed]
PHST- 1987/09/01 00:01 [medline]
PHST- 1987/09/01 00:00 [entrez]
AID - 0166-0934(87)90134-0 [pii]
AID - 10.1016/0166-0934(87)90134-0 [doi]
PST - ppublish
SO  - J Virol Methods. 1987 Sep;17(3-4):237-45. doi: 10.1016/0166-0934(87)90134-0.