PMID- 2445816
OWN - NLM
STAT- MEDLINE
DCOM- 19880112
LR  - 20191210
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 139
IP  - 11
DP  - 1987 Dec 1
TI  - The ability of cytotoxic T cells to recognize HLA-A2.1 or HLA-B7 antigens 
      expressed on murine cells correlates with their epitope specificity.
PG  - 3614-21
AB  - Two groups of human and murine cytotoxic T lymphocyte (CTL) clones specific for 
      human leukocyte antigen (HLA)-A2 or -B7 can be distinguished based on their 
      ability to kill murine transfectants expressing these molecules. The clones which 
      do not recognize murine transfectants exhibited greatly reduced conjugate 
      formation with these cells, indicating that the inability to lyse these cells 
      occurs in recognition and binding. No systematic differences in inhibitory titer 
      between the two types of CTL clones were seen with anti-CD8 (Lyt-2), anti-LFA-1, 
      or monoclonal antibodies against HLA class I molecules. However, blocking with 
      anti-HLA class I monoclonal antibodies suggested that different CTL clones 
      recognized spatially separate epitopes on HLA-A2 and -B7. In addition, a 
      correlation between the inability to recognize murine transfectants and fine 
      specificity was seen. Eight of nine clones which did not lyse murine 
      transfectants also failed to recognize human cells expressing HLA-A2.2 or -A2.3. 
      In contrast only 5 of 12 clones which lysed transfectants failed to recognize the 
      variant molecules. Analogous data were obtained with human CTL clones raised 
      against HLA-A2.1. These findings suggest that CTL clones that do not lyse murine 
      cells expressing appropriate antigens recognize epitopes that have been altered 
      or lost as a consequence of expression on the murine cell surface. It is 
      suggested that the loss of HLA-associated epitopes on the murine cell surface may 
      be due to differences between mouse and human cells in the processing or 
      presentation of class I-associated peptides.
FAU - Bernhard, E J
AU  - Bernhard EJ
AD  - Department of Microbiology, University of Virginia School of Medicine, 
      Charlottesville 22908.
FAU - Le, A X
AU  - Le AX
FAU - Yannelli, J R
AU  - Yannelli JR
FAU - Holterman, M J
AU  - Holterman MJ
FAU - Hogan, K T
AU  - Hogan KT
FAU - Parham, P
AU  - Parham P
FAU - Engelhard, V H
AU  - Engelhard VH
LA  - eng
GR  - AI20963/AI/NIAID NIH HHS/United States
GR  - AI21393/AI/NIAID NIH HHS/United States
GR  - CA00835/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (Recombinant Proteins)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Animals
MH  - Clone Cells/immunology
MH  - Epitopes/immunology
MH  - HLA Antigens/*immunology
MH  - HLA-A2 Antigen
MH  - HLA-B27 Antigen
MH  - L Cells/immunology
MH  - Mice
MH  - Mice, Inbred C57BL/immunology
MH  - Recombinant Proteins/immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Transfection
MH  - beta 2-Microglobulin/immunology
EDAT- 1987/12/01 00:00
MHDA- 1987/12/01 00:01
CRDT- 1987/12/01 00:00
PHST- 1987/12/01 00:00 [pubmed]
PHST- 1987/12/01 00:01 [medline]
PHST- 1987/12/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1987 Dec 1;139(11):3614-21.