PMID- 24458260
OWN - NLM
STAT- MEDLINE
DCOM- 20141011
LR  - 20211021
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 83
DP  - 2014 Jan 14
TI  - Videomorphometric analysis of hypoxic pulmonary vasoconstriction of 
      intra-pulmonary arteries using murine precision cut lung slices.
PG  - e50970
LID - 10.3791/50970 [doi]
LID - 50970
AB  - Acute alveolar hypoxia causes pulmonary vasoconstriction (HPV) - also known as 
      von Euler-Liljestrand mechanism - which serves to match lung perfusion to 
      ventilation. Up to now, the underlying mechanisms are not fully understood. The 
      major vascular segment contributing to HPV is the intra-acinar artery. This 
      vessel section is responsible for the blood supply of an individual acinus, which 
      is defined as the portion of lung distal to a terminal bronchiole. Intra-acinar 
      arteries are mostly located in that part of the lung that cannot be selectively 
      reached by a number of commonly used techniques such as measurement of the 
      pulmonary artery pressure in isolated perfused lungs or force recordings from 
      dissected proximal pulmonary artery segments(1,2). The analysis of subpleural 
      vessels by real-time confocal laser scanning luminescence microscopy is limited 
      to vessels with up to 50 microm in diameter(3). We provide a technique to study HPV 
      of murine intra-pulmonary arteries in the range of 20-100 microm inner diameters. It 
      is based on the videomorphometric analysis of cross-sectioned arteries in 
      precision cut lung slices (PCLS). This method allows the quantitative measurement 
      of vasoreactivity of small intra-acinar arteries with inner diameter between 
      20-40 microm which are located at gussets of alveolar septa next to alveolar ducts 
      and of larger pre-acinar arteries with inner diameters between 40-100 microm which 
      run adjacent to bronchi and bronchioles. In contrast to real-time imaging of 
      subpleural vessels in anesthetized and ventilated mice, videomorphometric 
      analysis of PCLS occurs under conditions free of shear stress. In our 
      experimental model both arterial segments exhibit a monophasic HPV when exposed 
      to medium gassed with 1% O2 and the response fades after 30-40 min at hypoxia.
FAU - Paddenberg, Renate
AU  - Paddenberg R
AD  - Institute of Anatomy and Cell Biology, Justus-Liebig-University; 
      renate.paddenberg@anatomie.med.uni-giessen.de.
FAU - Mermer, Petra
AU  - Mermer P
AD  - Institute of Anatomy and Cell Biology, Justus-Liebig-University.
FAU - Goldenberg, Anna
AU  - Goldenberg A
AD  - Institute of Anatomy and Cell Biology, Justus-Liebig-University.
FAU - Kummer, Wolfgang
AU  - Kummer W
AD  - Institute of Anatomy and Cell Biology, Justus-Liebig-University.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20140114
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Hypoxia/*physiopathology
MH  - Lung/anatomy & histology/*blood supply
MH  - Mice
MH  - Microscopy, Confocal/*methods
MH  - Pulmonary Alveoli/physiopathology
MH  - Pulmonary Artery/anatomy & histology/physiopathology
MH  - Vasoconstriction/physiology
MH  - Video Recording/*methods
PMC - PMC4089409
EDAT- 2014/01/25 06:00
MHDA- 2014/10/12 06:00
PMCR- 2014/01/14
CRDT- 2014/01/25 06:00
PHST- 2014/01/25 06:00 [entrez]
PHST- 2014/01/25 06:00 [pubmed]
PHST- 2014/10/12 06:00 [medline]
PHST- 2014/01/14 00:00 [pmc-release]
AID - 50970 [pii]
AID - 10.3791/50970 [doi]
PST - epublish
SO  - J Vis Exp. 2014 Jan 14;(83):e50970. doi: 10.3791/50970.