PMID- 24458608
OWN - NLM
STAT- MEDLINE
DCOM- 20150714
LR  - 20161125
IS  - 1864-0648 (Electronic)
IS  - 1864-063X (Linking)
VI  - 7
IP  - 11-12
DP  - 2014 Nov
TI  - Evaluation of ultrasound and glucose synergy effect on the optical clearing and 
      light penetration for human colon tissue using SD-OCT.
PG  - 938-47
LID - 10.1002/jbio.201300141 [doi]
AB  - Topical application optical clearing agents (OCAs) can effectively enhance the 
      tissue optical clearing on the human colon tissue, which has been demonstrated in 
      our previous studies. Nevertheless, the strong light scattering still limits the 
      diffusion rate of OCAs and penetration depth of light into the tissue. In this 
      study, in order to further increase the diffusion of the OCA of glucose into 
      tissue, we employ a method to improve the glucose permeability and light 
      penetration with ultrasound (sonophoretic delivery, SP) and glucose (G) synergy 
      on human normal and cancerous colon tissues in vitro, which was measured and 
      quantified with spectral-domain optical coherence tomography (SD-OCT) technology. 
      To evaluate the effect of ultrasound mediation, the percentages of OCT signal 
      enhancement (PSE) and 1/e light-penetration depth were calculated for G alone and 
      ultrasound-G treatments. The PSE was calculated at approximately 313 mum from the 
      sample tissue surface. For normal and cancerous colon tissues the PSE were about 
      91.1 +/- 10.6% and 65.3% +/- 12.3% with 30% G/SP, but for the 30% G alone treatment 
      it was about 78.6 +/- 11.2% and 54.5% +/- 9.3%, respectively. The max value of 1/e 
      light-penetration depth for normal colon tissue was 0.47 +/- 0.02 mm with 30% G 
      alone and 0.60 +/- 0.05 mm (p < 0.05)with 30% G/SP synergy. However, for the 
      cancerous colon tissue the max value was 0.45 +/- 0.01 mm and 0.57 +/- 0.03 mm (p < 
      0.05), respectively. The obtained permeability coefficients showed a significant 
      enhancement with ultrasound mediation. The mean permeability coefficients of 30% 
      G/SP in normal and cancerous colon tissues were (6.3 +/- 0.16) x 10(-6) cm/s and 
      (12.1 +/- 0.34) x 10(-6) cm/s (p < 0.05), respectively. These preliminary 
      experiments showed that ultrasound can effectively enhance the tissue optical 
      clearing and glucose diffusion rate as well as increase the light-penetration 
      depth into biotissues.
CI  - Copyright (c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Zhao, Qingliang
AU  - Zhao Q
AD  - School of Biomedical Engineering, Shanghai Jiao Tong University, No. 800 
      Dongchuan Road, Shanghai 200240, China.
FAU - Wei, Huajiang
AU  - Wei H
FAU - He, Yonghong
AU  - He Y
FAU - Ren, Qiushi
AU  - Ren Q
FAU - Zhou, Chuanqing
AU  - Zhou C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140124
PL  - Germany
TA  - J Biophotonics
JT  - Journal of biophotonics
JID - 101318567
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Colon/*diagnostic imaging/*drug effects/pathology
MH  - Colonic Neoplasms/*diagnostic imaging/pathology
MH  - Diffusion
MH  - Glucose/*chemistry
MH  - Humans
MH  - Light
MH  - Mice
MH  - Optics and Photonics
MH  - Permeability
MH  - Signal Processing, Computer-Assisted
MH  - Skin/drug effects
MH  - Temperature
MH  - Tomography, Optical Coherence/methods
MH  - Ultrasonography
OTO - NOTNLM
OT  - colon cancer
OT  - hyperosmotic agent
OT  - light-penetration depth
OT  - optical coherence tomography
OT  - permeability coefficient
OT  - ultrasound
EDAT- 2014/01/25 06:00
MHDA- 2015/07/15 06:00
CRDT- 2014/01/25 06:00
PHST- 2013/09/01 00:00 [received]
PHST- 2013/11/18 00:00 [revised]
PHST- 2013/12/02 00:00 [accepted]
PHST- 2014/01/25 06:00 [entrez]
PHST- 2014/01/25 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - 10.1002/jbio.201300141 [doi]
PST - ppublish
SO  - J Biophotonics. 2014 Nov;7(11-12):938-47. doi: 10.1002/jbio.201300141. Epub 2014 
      Jan 24.