PMID- 24459064
OWN - NLM
STAT- MEDLINE
DCOM- 20140624
LR  - 20240109
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 35
IP  - 5
DP  - 2014 May
TI  - Genomic characterization of three urinary bladder cancer cell lines: 
      understanding genomic types of urinary bladder cancer.
PG  - 4599-617
LID - 10.1007/s13277-013-1604-3 [doi]
AB  - Several genomic regions are frequently altered and associated with the type, 
      stage and progression of urinary bladder cancer (UBC). We present the 
      characterization of 5637, T24 and HT1376 UBC cell lines by karyotyping, 
      fluorescence in situ hybridization (FISH), array comparative genomic 
      hybridization (aCGH) and multiplex ligation-dependent probe amplification (MLPA) 
      analysis. Some cytogenetic anomalies present in UBC were found in the three cell 
      lines, such as chromosome 20 aneuploidy and the loss of 9p21. Some gene loci 
      losses (e.g. CDKN2A) and gains (e.g. HRAS, BCL2L1 and PTPN1) were coincident 
      across all cell lines. Although some significant heterogeneity and complexity 
      were detected between them, their genomic profiles exhibited a similar pattern to 
      UBC. We suggest that 5637 and HT1376 represent the E2F3/RB1 pathway due to 
      amplification of 6p22.3, concomitant with loss of one copy of RB1 and mutation of 
      the remaining copy. The HT1376 presented a 10q deletion involving PTEN region and 
      no alteration of PIK3CA region which, in combination with the inactivation of 
      TP53, bears more invasive and metastatic properties than 5637. The T24 belongs to 
      the alternative pathway of FGFR3/CCND1 by presenting mutated HRAS and 
      over-represented CCND1. These cell lines cover the more frequent subtypes of UBC 
      and are reliable models that can be used, as a group, in preclinical studies.
FAU - Pinto-Leite, Rosario
AU  - Pinto-Leite R
AD  - Cytogenetic Laboratory, Department of Human Genetics, Hospital Center of 
      Tras-os-Montes and Alto Douro, Vila Real, Portugal.
FAU - Carreira, Isabel
AU  - Carreira I
FAU - Melo, Joana
AU  - Melo J
FAU - Ferreira, Susana Isabel
AU  - Ferreira SI
FAU - Ribeiro, Ilda
AU  - Ribeiro I
FAU - Ferreira, Jaqueline
AU  - Ferreira J
FAU - Filipe, Marco
AU  - Filipe M
FAU - Bernardo, Carina
AU  - Bernardo C
FAU - Arantes-Rodrigues, Regina
AU  - Arantes-Rodrigues R
FAU - Oliveira, Paula
AU  - Oliveira P
FAU - Santos, Lucio
AU  - Santos L
LA  - eng
PT  - Journal Article
DEP - 20140124
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
SB  - IM
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Comparative Genomic Hybridization
MH  - Disease Progression
MH  - Drug Resistance, Multiple
MH  - Female
MH  - Genes, Retinoblastoma
MH  - Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 2014/01/25 06:00
MHDA- 2014/06/25 06:00
CRDT- 2014/01/25 06:00
PHST- 2013/11/19 00:00 [received]
PHST- 2013/12/30 00:00 [accepted]
PHST- 2014/01/25 06:00 [entrez]
PHST- 2014/01/25 06:00 [pubmed]
PHST- 2014/06/25 06:00 [medline]
AID - 10.1007/s13277-013-1604-3 [doi]
PST - ppublish
SO  - Tumour Biol. 2014 May;35(5):4599-617. doi: 10.1007/s13277-013-1604-3. Epub 2014 
      Jan 24.