PMID- 24459475
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140124
LR  - 20211021
IS  - 1735-5362 (Print)
IS  - 1735-9414 (Electronic)
IS  - 1735-5362 (Linking)
VI  - 8
IP  - 1
DP  - 2013 Jan
TI  - Comparison of bleomycin-induced pulmonary apoptosis between NMRI mice and C57BL/6 
      mice.
PG  - 43-50
AB  - Apoptosis has a critical role in the pathogenesis of bleomycin induced-pulmonary 
      fibrosis. The severity of fibrosis varies among different strains of mice. Recent 
      studies have indicated that expression of apoptotic regulatory genes may be 
      specific in different cell types in various strains. In this study, 
      bleomycin-induced pulmonary apoptosis in NMRI (Naval Medical Research Institute, 
      USA) albino mice were compared with C57BL/6 black mice. Pulmonary fibrosis 
      induced by single intratracheal administration of bleomycin (3 U/kg). Control 
      mice were instilled with the same volume of saline. After 2 weeks, fibrotic 
      responses were studied by biochemical measurement of collagen deposition and 
      histological examination of pathological lung changes. Apoptosis was detected and 
      quantitated by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) assay. Bleomycin significantly (P<0.05) increased lung collagen 
      content and also induced fibrotic histological changes in both strains. Apoptosis 
      was detected in the bronchiolar and alveolar epithelial cells after bleomycin 
      instillation. TUNEL-positive alveolar epithelial cells in bleomycin-treated lungs 
      of C57BL/6 and NMRI mice (19.5% + 2.7 and 17% + 2.0, respectively) were 
      significantly (P<0.05) higher than that of saline-treated lungs (1.5% + 0.5) with 
      no significant difference between two strains of mice (P>0.05). Despite some 
      murine strain variation in the expression of apoptotic regulatory genes in 
      bleomycin-induced pulmonary fibrosis, the results of the present study revealed 
      no significant differences in alveolar epithelial apoptosis between NMRI and 
      C57BL/6 black mice. However, these results confirm the role of apoptosis in the 
      pathogenesis of pulmonary fibrosis and suitability of both strains as 
      experimental models of lung fibrosis.
FAU - Safaeian, L
AU  - Safaeian L
AD  - Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences 
      Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan 
      University of Medical Sciences, Isfahan, I.R. Iran.
FAU - Jafarian-Dehkordi, A
AU  - Jafarian-Dehkordi A
AD  - Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences 
      Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan 
      University of Medical Sciences, Isfahan, I.R. Iran.
FAU - Rabbani, M
AU  - Rabbani M
AD  - Department of Pharmacology and Toxicology and Isfahan Pharmaceutical Sciences 
      Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan 
      University of Medical Sciences, Isfahan, I.R. Iran.
FAU - Sadeghi, H M
AU  - Sadeghi HM
AD  - Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical 
      Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
FAU - Afshar-Moghaddam, N
AU  - Afshar-Moghaddam N
AD  - Department of Pathology, School of Medicine, Isfahan University of Medical 
      Sciences, Isfahan, I.R. Iran.
FAU - Sarahroodi, S
AU  - Sarahroodi S
AD  - Department of pharmacology, School of Medicine, Qom University of Medical 
      Sciences, Qom, I.R. Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Res Pharm Sci
JT  - Research in pharmaceutical sciences
JID - 101516968
PMC - PMC3895299
OTO - NOTNLM
OT  - Apoptosis
OT  - Bleomycin
OT  - C57BL/6 mice
OT  - NMRI mice
OT  - Pulmonary fibrosis
EDAT- 2014/01/25 06:00
MHDA- 2014/01/25 06:01
PMCR- 2013/01/01
CRDT- 2014/01/25 06:00
PHST- 2014/01/25 06:00 [entrez]
PHST- 2014/01/25 06:00 [pubmed]
PHST- 2014/01/25 06:01 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - JRPS-8-43 [pii]
PST - ppublish
SO  - Res Pharm Sci. 2013 Jan;8(1):43-50.