PMID- 24460735
OWN - NLM
STAT- MEDLINE
DCOM- 20150402
LR  - 20221207
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 18
IP  - 3
DP  - 2014 Mar
TI  - Engineering of pre-vascularized urethral patch with muscle flaps and 
      hypoxia-activated hUCMSCs improves its therapeutic outcome.
PG  - 434-43
LID - 10.1111/jcmm.12157 [doi]
AB  - Tissue engineering has brought new hopes for urethral reconstruction. However, 
      the absence of pre-vascularization and the subsequent degradation of materials 
      often lead to the failure of in vivo application. In this study, with the 
      assistance of hypoxia-activated human umbilical cord mesenchymal stem cells 
      (hUCMSCs), pedicled muscle flaps were used as materials and pre-incubated in 
      ventral penile subcutaneous cavity of rabbit for 3 weeks to prepare a 
      pre-vascularized urethral construct. We found that small vessels and muscle 
      fibres were scattered in the construct after 3 weeks' pre-incubation. The 
      construct presented a fibrous reticular structure, which was similar to that of 
      the corpus spongiosum under microscope examination. The produced constructs were 
      then used as a patch graft for reconstruction of the defective rabbit urethra 
      (experimental group), natural muscular patch was used as control (control group). 
      Twelve weeks after the reconstructive surgery, urethrography and urethroscope 
      inspections showed wide calibres of the reconstructed urethra in the experimental 
      group. Histopathological studies revealed that fibrous connective tissues and 
      abundant muscle fibres constituted the main body of the patch-grafted urethra. In 
      contrast, in the control group, only adipose tissue was found in the 
      stenosis-reconstructed urethra, replacing the originally grafted muscular tissue. 
      To our knowledge, this is the first report that successfully constructed a 
      pre-vascularized urethral construct by using hypoxia-activated hUCMSC and 
      pedicled muscle flaps. More importantly, the pre-vascularized construct showed a 
      good performance in urethral reconstruction when applied in vivo. The study 
      provided a novel strategy for tissue engineering of pre-vascularized urethral 
      construct for the defective urethra, representing a further advancement in 
      urethral reconstruction.
CI  - (c) 2013 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - Sun, Dongchong
AU  - Sun D
AD  - Department of Urology, People's Liberation Army General Hospital, Beijing, China.
FAU - Yang, Yong
AU  - Yang Y
FAU - Wei, Zhitao
AU  - Wei Z
FAU - Xu, Yong
AU  - Xu Y
FAU - Zhang, Xu
AU  - Zhang X
FAU - Hong, Baofa
AU  - Hong B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140125
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Cytokines)
SB  - IM
MH  - Angiogenesis Inducing Agents/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Cell Hypoxia
MH  - Cell Separation
MH  - Cytokines/metabolism
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Muscles/*blood supply
MH  - Rabbits
MH  - Radiography
MH  - Plastic Surgery Procedures
MH  - Subcutaneous Tissue/physiology
MH  - Surgical Flaps/*blood supply
MH  - Tissue Engineering/*methods
MH  - Umbilical Cord/*cytology
MH  - Urethra/*blood supply/diagnostic imaging/surgery
PMC - PMC3955150
OTO - NOTNLM
OT  - hUCMSC
OT  - pedicled muscle flaps
OT  - tissue engineering
OT  - urethral reconstruction
EDAT- 2014/01/28 06:00
MHDA- 2015/04/04 06:00
PMCR- 2014/03/01
CRDT- 2014/01/28 06:00
PHST- 2013/05/17 00:00 [received]
PHST- 2013/09/09 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2015/04/04 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - 10.1111/jcmm.12157 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2014 Mar;18(3):434-43. doi: 10.1111/jcmm.12157. Epub 2014 Jan 25.