PMID- 24461004
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20140127
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 82
IP  - 4
DP  - 2013 Oct
TI  - HLA-A, -B, -C, -DRB1 and -DQB1 allele and haplotype frequencies in Saudis using 
      next generation sequencing technique.
PG  - 252-8
LID - 10.1111/tan.12200 [doi]
AB  - Next generation sequencing (NGS) is a promising technique that can reveal the 
      entire gene sequences and to the highest possible resolution without any phase 
      ambiguities. We have used this technique to investigate the frequencies of HLA-A, 
      -B, -C, -DRB1 and -DQB1 in a Saudi cohort of healthy individuals. We used NGS 
      using the 454 genome sequence (GS) FLX System and Conexio assign atf 454 software 
      to human leukocyte antigen (HLA) genotype eight class I and class II loci. A 
      total of 158 healthy Saudi adults were analyzed. The most frequently observed 
      allele for HLA-A was HLA-A*02:01:01:01 (13.6%); for HLA-B, HLA-B*50:01:01 
      (15.8%); for HLA-C, HLA-C*06:02:01:01 (18.7%); for HLA-DRB1, HLA-DRB1*07:01:01:01 
      (26.6%); and for HLA-DQB1, HLA-DQB1*02:01:01 (20.3%). The most common four loci 
      haplotypes in the Saudi population were 
      HLA-A*24:02:01:01-B*08:01:01-C*07:02:01:01-DRB1*03:01:01:01 and 
      HLA-A*23:01:01-B*50:01:01-C*06:02:01:01-DRB1*07:01:01:01.. We have used a highly 
      informative technique for HLA typing of a Saudi healthy cohort to establish 
      allele and haplotype frequencies. These results should prove useful for 
      population studies, disease associations and future planning of the unrelated 
      bone marrow donor registry.
CI  - (c) 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Hajeer, A H
AU  - Hajeer AH
AD  - Department of Pathology & Laboratory Medicine, King Abdulaziz Medical City, 
      National Guard Health Affairs, Riyadh, Kingdom of Saudi Arabia; College of 
      Medicine, King Saud bin Abdulaziz University for Health Sciences, National Guard 
      Health Affairs, Riyadh, Kingdom of Saudi Arabia.
FAU - Al Balwi, M A
AU  - Al Balwi MA
FAU - Aytul Uyar, F
AU  - Aytul Uyar F
FAU - Alhaidan, Y
AU  - Alhaidan Y
FAU - Alabdulrahman, A
AU  - Alabdulrahman A
FAU - Al Abdulkareem, I
AU  - Al Abdulkareem I
FAU - Al Jumah, M
AU  - Al Jumah M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alleles
MH  - Female
MH  - Gene Frequency
MH  - HLA-A Antigens/*immunology
MH  - HLA-B Antigens/*immunology
MH  - HLA-C Antigens/*immunology
MH  - HLA-DQ beta-Chains/*immunology
MH  - HLA-DRB1 Chains/*immunology
MH  - Haplotypes
MH  - High-Throughput Nucleotide Sequencing
MH  - Histocompatibility Testing
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Saudi Arabia
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Saudi
OT  - human leukocyte antigen
OT  - next generation sequencing
OT  - population genetics
EDAT- 2014/01/28 06:00
MHDA- 2014/09/16 06:00
CRDT- 2014/01/28 06:00
PHST- 2013/05/19 00:00 [received]
PHST- 2013/08/01 00:00 [revised]
PHST- 2013/08/24 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
AID - 10.1111/tan.12200 [doi]
PST - ppublish
SO  - Tissue Antigens. 2013 Oct;82(4):252-8. doi: 10.1111/tan.12200.