PMID- 24461061
OWN - NLM
STAT- MEDLINE
DCOM- 20140924
LR  - 20220321
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 58
IP  - 6
DP  - 2014 Jun
TI  - Anticancer effects of pyocyanin on HepG2 human hepatoma cells.
PG  - 541-8
LID - 10.1111/lam.12224 [doi]
AB  - Pyocyanin, a major virulence factor produced by Pseudomonas aeruginosa, displays 
      redox activity and damaging effects on mammalian cells. In this study, we 
      investigated the effects of pyocyanin on the proliferation of HepG2 tumour cells. 
      Interestingly, pyocyanin significantly inhibited cell proliferation and triggered 
      the production of large amounts of reactive oxygen species (ROS), thereby 
      upregulating superoxide dismutase (SOD) and catalase (CAT). Additionally, 
      pyocyanin treatment significantly depleted reduced glutathione (GSH) and 
      decreased the GSH/oxidized GSH (GSSG) ratio. These results supported that 
      pyocyanin-induced cytotoxicity in HepG2 cells was mediated by acute ROS 
      production and subsequent oxidative stress. SA-beta-Gal, acridine orange 
      (AO)/ethidium bromide (EB) double staining, caspase-3 measurements and comet 
      assay results revealed that cell death induced by pyocyanin involved DNA damage 
      and activation of caspase-3, accelerating cell senescence and apoptosis. Thus, 
      our data provided insights into the mechanisms underlying pyocyanin-induced 
      cytotoxicity and may lead to better treatment strategies for cancer. SIGNIFICANCE 
      AND IMPACT OF THE STUDY: Pyocyanin is a redox-active phenazine toxin. Here, we 
      investigated the ability of pyocyanin to inhibit cancer-related phenotypes in 
      HepG2 human hepatoma cells. Our results indicated that pyocyanin accelerated 
      cellular senescence and apoptosis and induced oxidative stress-associated DNA 
      damage in HepG2 cells. The potential anticancer applications of pyocyanin should 
      be investigated further in clinical studies.
CI  - (c) 2014 The Society for Applied Microbiology Published 2014. This article is a 
      U.S. Government work and is in the public domain in the USA.
FAU - Zhao, J
AU  - Zhao J
AD  - School of Environment & Biological Engineering, Nanjing University of Science & 
      Technology, Nanjing, China.
FAU - Wu, Y
AU  - Wu Y
FAU - Alfred, A T
AU  - Alfred AT
FAU - Wei, P
AU  - Wei P
FAU - Yang, S
AU  - Yang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140307
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 9OQM399341 (Pyocyanine)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Carcinoma, Hepatocellular
MH  - Catalase/metabolism
MH  - Cell Proliferation/drug effects
MH  - Drug Screening Assays, Antitumor
MH  - Glutathione/metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Inhibitory Concentration 50
MH  - Liver Neoplasms
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Pyocyanine/*pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - HepG2
OT  - apoptosis
OT  - oxidative stress
OT  - pyocyanin
OT  - reactive oxygen species
OT  - senescence
EDAT- 2014/01/28 06:00
MHDA- 2014/09/25 06:00
CRDT- 2014/01/28 06:00
PHST- 2013/08/28 00:00 [received]
PHST- 2014/01/20 00:00 [revised]
PHST- 2014/01/20 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/09/25 06:00 [medline]
AID - 10.1111/lam.12224 [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2014 Jun;58(6):541-8. doi: 10.1111/lam.12224. Epub 2014 Mar 
      7.