PMID- 24461183
OWN - NLM
STAT- MEDLINE
DCOM- 20140605
LR  - 20140127
IS  - 1008-8830 (Print)
IS  - 1008-8830 (Linking)
VI  - 16
IP  - 1
DP  - 2014 Jan
TI  - [Effect of integrin beta8 on TGF-beta1 activation in astrocytes with oxygen glucose 
      deprivation].
PG  - 73-6
AB  - OBJECTIVE: To study the effect of beta8 expression on transforming growth factor 
      beta1(TGF-beta1) activation in astrocytes with oxygen glucose deprivation (OGD). 
      METHODS: Astrocytes were cultured and then subjected to OGD to generate 
      hypoxia-ischemia (HI) model in vitro. Immunocytochemistry was used to detect the 
      expression and distribution of beta8 in nomoxia cultured cells. beta8 protein 
      expression was quantified by Western blot at 12 hours, 1 day and 2 days after 
      OGD. Astrocytes and luciferase reporter cells (TMLC) were co-cultured. beta8 RNA 
      interference system was established to specifically inhibit beta8 expression in 
      cultured astrocytes. TGF-beta1 activation was then detected in the co-culture 
      system. RESULTS: beta8 was mainly located in the cytoplasm and neurites of 
      astrocytes. OGD resulted in increase of beta8 protein expression at 12 hours after 
      reoxygenation in astrocytes, which was peaked at 1 day after reoxygenation. 
      TGF-beta1 activation was in accordance with beta8 expression in astrocyte-TMLC 
      co-culture system after reoxygenation. After the inhibition of beta8, TGF-beta1 
      activation was significantly reduced in all time points. CONCLUSIONS: The highly 
      expressed beta8 plays important roles in the regulation of TGF-beta1 activation in 
      neonatal rats with hypoxic-ischemic brain damage.
FAU - Li, Jin-Hui
AU  - Li JH
AD  - Department of Pediatrics, West China Second Hospital, Sichuan University; West 
      China Children's Medical Center; Key Laboratory of Obstetric & Gynecologic and 
      Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu 610041, 
      China. quyi712002@yahoo.com.cn.
FAU - Li, De-Yuan
AU  - Li DY
FAU - Chen, Da-Peng
AU  - Chen DP
FAU - Mu, De-Zhi
AU  - Mu DZ
FAU - Qu, Yi
AU  - Qu Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Dang Dai Er Ke Za Zhi
JT  - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
JID - 100909956
RN  - 0 (Integrin beta Chains)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (integrin beta8)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Female
MH  - Glucose/metabolism
MH  - Hypoxia-Ischemia, Brain/*metabolism
MH  - Integrin beta Chains/*physiology
MH  - Male
MH  - Oxygen/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta1/*metabolism
EDAT- 2014/01/28 06:00
MHDA- 2014/06/06 06:00
CRDT- 2014/01/28 06:00
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/06/06 06:00 [medline]
AID - 10.7499/j.issn.1008-8830.2014.01.016 [pii]
PST - ppublish
SO  - Zhongguo Dang Dai Er Ke Za Zhi. 2014 Jan;16(1):73-6.