PMID- 24462543
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20200106
IS  - 1873-474X (Electronic)
IS  - 0736-5748 (Linking)
VI  - 34
DP  - 2014 May
TI  - Maternal micronutrient imbalance alters gene expression of BDNF, NGF, TrkB and 
      CREB in the offspring brain at an adult age.
PG  - 24-32
LID - S0736-5748(14)00017-3 [pii]
LID - 10.1016/j.ijdevneu.2014.01.003 [doi]
AB  - Micronutrients like folate, vitamin B12, and fatty acids which are interlinked in 
      the one carbon cycle play a vital role in mediating epigenetic processes leading 
      to an increased risk for neurodevelopmental disorders in the offspring. Our 
      earlier study demonstrates that a micronutrient imbalanced diet adversely affects 
      docosahexaenoic acid (DHA) and protein levels of neurotrophins like brain-derived 
      neurotrophic factor (BDNF) and nerve growth factor (NGF) in the brain and 
      cognition in the offspring by 3 months of age. In this study we attempt to 
      analyze if these effects are a consequence of a change in gene expression of 
      these molecules. Further, we also examined the effect of either a postnatal 
      control diet or a prenatal omega-3 fatty acid supplementation on gene expression 
      in the cortex of the offspring. Pregnant rats were divided into control and five 
      treatment groups at two levels of folic acid (normal and excess folate) in the 
      presence and absence of vitamin B12. Omega-3 fatty acid (eicosapentaenoic acid - 
      EPA+DHA) supplementation was given to vitamin B12 deficient groups. Following 
      delivery, 8 dams from each group were shifted to control diet and remaining 
      continued on the same treatment diet. Our results demonstrate that the imbalanced 
      diet caused a marked reduction in the mRNA levels of BDNF, NGF, TrkB, and cAMP 
      response element-binding protein (CREB). Prenatal omega-3 fatty acid 
      supplementation to the maternal imbalanced diet was able to normalize the mRNA 
      levels of all the above genes. This study demonstrates that a maternal diet 
      imbalanced in micronutrients (folic acid, vitamin B12) influences gene expression 
      of neurotrophins and their signalling molecules and thereby adversely affects the 
      brain of the offspring.
CI  - Copyright (c) 2014 ISDN. Published by Elsevier Ltd. All rights reserved.
FAU - Sable, Pratiksha
AU  - Sable P
AD  - Department of Nutritional Medicine, Interactive Research School for Health 
      Affairs, Bharati Vidyapeeth Deemed University, Pune Satara Road, Pune 411043, 
      India.
FAU - Kale, Anvita
AU  - Kale A
AD  - Department of Nutritional Medicine, Interactive Research School for Health 
      Affairs, Bharati Vidyapeeth Deemed University, Pune Satara Road, Pune 411043, 
      India.
FAU - Joshi, Asmita
AU  - Joshi A
AD  - Department of Nutritional Medicine, Interactive Research School for Health 
      Affairs, Bharati Vidyapeeth Deemed University, Pune Satara Road, Pune 411043, 
      India.
FAU - Joshi, Sadhana
AU  - Joshi S
AD  - Department of Nutritional Medicine, Interactive Research School for Health 
      Affairs, Bharati Vidyapeeth Deemed University, Pune Satara Road, Pune 411043, 
      India. Electronic address: srjoshi62@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140122
PL  - United States
TA  - Int J Dev Neurosci
JT  - International journal of developmental neuroscience : the official journal of the 
      International Society for Developmental Neuroscience
JID - 8401784
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Micronutrients)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - 935E97BOY8 (Folic Acid)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/growth & development/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cyclic AMP Response Element-Binding Protein/genetics/*metabolism
MH  - Fatty Acids, Omega-3/administration & dosage
MH  - Female
MH  - Folic Acid/administration & dosage
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Male
MH  - Micronutrients/*administration & dosage
MH  - Nerve Growth Factor/genetics/*metabolism
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
OTO - NOTNLM
OT  - BDNF
OT  - CREB
OT  - Cognition
OT  - Micronutrient imbalance
OT  - NGF
OT  - TrkB
EDAT- 2014/01/28 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/01/28 06:00
PHST- 2013/12/06 00:00 [received]
PHST- 2014/01/07 00:00 [revised]
PHST- 2014/01/13 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0736-5748(14)00017-3 [pii]
AID - 10.1016/j.ijdevneu.2014.01.003 [doi]
PST - ppublish
SO  - Int J Dev Neurosci. 2014 May;34:24-32. doi: 10.1016/j.ijdevneu.2014.01.003. Epub 
      2014 Jan 22.